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[null,"KAPA Library Quantification Kit (Illumina® platforms)",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
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[null,"Chromium Connect Library Quantification Worksheet"]
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[null,"This spreadsheet is a modification of the Data Analysis Template provided by KAPA Biosciences for analysis of NGS library quantification data generated with the KAPA Library Quantification Kit (Illumina® platforms). It has been adjusted to include format-specific settings and information for using the KAPA Library Quantification Kit on the 10x Genomics Chromium Connect instrument."]
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[null,"For information on the complete Chromium Connect protocol, consult the Chromium Connect Instrument User Guide (CG000180) and the Chromium Next GEM Automated Single Cell 3' Reagent Kits v3.1 User Guide (CG000286)"]
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[null,"The \"Chromium Connect Assay Info\" tab in this Excel sheet collects sample names and library sizes for a given Chromium Connect Assay. "]
[null,"The \"Cq Data Input\" tab collects Cq information for each sample generated by the quantification kit. The \"Analysis\" and \"Summary\" tabs analyze and summarize the data."]
[null,"The fourth sheet can also be used to calculate the yield of each quantified library by entering the library volumes in the designated column."]
[null,"Tabs marked \"example\" provide example data to show what final data analysis may look like."]
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[null,"Data analysis consists of three parts:"]
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[null,"1. Review Cq values for the six DNA Standards to evaluate the reliability of the data:"]
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[null,"This excel sheet will automatically import Cq values entered into \"Cq data input\" into relevant cells in the \"Analysis\" tab."]
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[null,"- If the Cq value for any replicate varies by more than +/- 0.25 of a cycle from the other value(s) (as calculated in column I), that replicate should"]
[null,"   be regarded as an outlier, and moved to column G. If a value is designated an outlier, delete the formula in the corresponding row of column I."]
[null,"  The difference of 0.25 cycles is arbitrary and instrument-dependent. When deciding on what to classify as an outlier, keep in mind that 1 cycle "]
[null,"  represents a 2-fold difference in concentration."]
[null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between"]
[null,"  3.2 and 3.45 is very good, whereas 3.1 - 3.6 is acceptable). If the spacing between any two standards is less than 3.1 cycles and more than "]
[null,"  3.6 cycles, the corresponding Delta Cq cell in column J will change to red. One or more red Delta Cq cells are an indication of unacceptable "]
[null,"  experimental variation. The assay will most likely not yield reliable library concentrations, and should be repeated. Please refer to the KAPA"]
[null,"  Library Quantification Kit Technical Data Sheet and the KAPA Library Quantification Technical Guide for detailed discussions related to "]
[null,"  experimental factors that can affect the reliability of the assay."]
[null,"- The Delta Cq between no template control (NTC) reactions and DNA Standard 6 should preferably be ≥3 cycles."]
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[null,"2. Generate and review the standard curve and reaction efficiency:"]
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[null,"Data from the previous section are used to generate the standard curve and calculate the amplification efficiency for the six DNA standards."]
[null,"Key parameters are calculated using two different methods, and guidelines for interpreting results are provided."]
[null,"- At least one and a maximum of two DNA Standards may be omitted from the standard curve (if Cq values for those Standards are deemed to"]
[null,"   be unreliable). This can be done by modifying the data range for the graph and formulae in cells D47, D48, D49 and D53. When omitting "]
[null,"   DNA Standards from the curve, remember that the curve may not be extrapolated, and concentrations of libraries with an average Cq value"]
[null,"   outside of the range of the (modified) standard curve may not be calculated."]
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[null,"3. Calculate library concentrations:"]
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[null,"The slope and intercept of the standard curve are used to calculate the concentrations of library samples. "]
[null,"  Please refer to Section 5 of the KAPA Library Quantification Kit Technical Data Sheet for complete guidelines."]
[null,"- If more than one dilution of a library was assayed, the Delta Cq column may be used to assess whether calculated concentrations are likely to"]
[null,"   be reliable. For example, if the Delta Cq for consecutive 2-fold dilutions falls outside the range of 0.9 - 1.1 cycles, it is an indication that serial "]
[null,"   dilutions were not made with a high degree of accuracy, and that calculated concentrations may be unreliable as a result."]
[null,"- If more than one dilution of a library was assayed, use the % Deviation column to determine the working concentration of the "]
[null,"   library. If the library concentrations calculated from multiple dilutions falls within 10% from one another, use the average of the concentrations."]
[null,"   If the calculated concentrations differ by more than 10%, use the average concentration from the dilutions that are deemed (most) reliable."]
[null,"   In such cases, modify the formula for calculating the working concentration accordingly. "]
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[null,"Calculated library concentrations are exported to the table in the Summary sheet following the Analysis sheet. The amount of each library"]
[null,"available for the next step in your workflow may be calculated by inserting library volumes in the designated column. This worksheet may be"]
[null,"extended and customized to calculate other library parameters (e.g. conversion rates, volumes needed for pooling) as per your specific requirements."]
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[null,"Readme text highlighted in aqua was written by 10x Genomics."]
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[null,"© 2020 10X Genomics, Inc. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.",null,null,null,null,null,null,null,null,null,null,null,"Contact:"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,"support@10xgenomics.com"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,"10x Genomics"]
[null,null,null,null,null,null,null,null,null,null,null,null,null,"6230 Stoneridge Mall Road "]
[null,null,null,null,null,null,null,null,null,null,null,null,null,"Pleasanton, CA 94588 USA"]

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[]
[]
[null,null,"Section 1. Review Cq values for DNA Standards"]
[]
[null,null,"- Enter the appropriate information into the fields highlighted in green."]
[null,null,"- Move  \"outliers\" to column G (so these are no longer is used in calculations). Delete the formula in the corresponding row in column I."]
[null,null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between 3.2 and 3.45 is very good  "]
[null,null,"   and 3.1 - 3.6 is acceptable)."]
[null,null,"- If the spacing between any two standards is less than 3.1 cycles and more than 3.6 cycles, those data points (and any library samples falling between those"]
[null,null,"  data points) are not highly reliable."]
[]
[]
[null,null,"Well","Std #","Conc (pM)","Cq","Outliers","Av Cq","Difference","Delta Cq"]
[null,null,null,1,20,null,null,{"$f":"AVERAGE(F14:F16)","v":{"$error":"#DIV/0!"}},{"$f":"F14-$H$14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,null,1,20,null,null,null,{"$f":"F15-$H$14","v":{"$error":"#DIV/0!"}}]
[null,null,null,1,20,null,null,null,{"$f":"F16-$H$14","v":{"$error":"#DIV/0!"}},{"$f":"H17-H14","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,{"$f":"AVERAGE(F17:F19)","v":{"$error":"#DIV/0!"}},{"$f":"F17-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F18-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F19-$H$17","v":{"$error":"#DIV/0!"}},{"$f":"H20-H17","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,{"$f":"AVERAGE(F20:F22)","v":{"$error":"#DIV/0!"}},{"$f":"F20-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F21-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F22-$H$20","v":{"$error":"#DIV/0!"}},{"$f":"H23-H20","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,{"$f":"AVERAGE(F23:F25)","v":{"$error":"#DIV/0!"}},{"$f":"F23-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F24-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F25-$H$23","v":{"$error":"#DIV/0!"}},{"$f":"H26-H23","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,{"$f":"AVERAGE(F26:F28)","v":{"$error":"#DIV/0!"}},{"$f":"F26-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F27-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F28-$H$26","v":{"$error":"#DIV/0!"}},{"$f":"H29-H26","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,{"$f":"AVERAGE(F29:F31)","v":{"$error":"#DIV/0!"}},{"$f":"F29-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F30-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F31-$H$29","v":{"$error":"#DIV/0!"}},{"$f":"H32-H29","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-",null,null,{"$f":"AVERAGE(F32:F34)","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-"]
[null,null,null,"NTC","-"]
[]
[]
[]
[]
[]
[null,null,"Section 2. Generate and review the standard curve"]
[]
[null,null,"- Type the value for the intercept from the graph to the right into cell D57."]
[null,null,"- Type the value for the slope from the graph to the right into cell D59. ",null,null,null,null,null,null,null,"Note: if the standard curve equation does not update, click on the line, right click and select"]
[null,null,null,null,null,null,null,null,null,null,"Format Trendline. (Untick and) tick the boxes for \"Display Equation and R-squared value on chart\""]
[]
[]
[null,null,"DNA Standard","Conc in pM","Log conc","Average Cq","Delta Cq"]
[null,null,1,20,{"$f":"LOG(D48)","v":1.3010299956639813},{"$f":"H14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,2,2,0.3010299956639812,{"$f":"H17","v":{"$error":"#DIV/0!"}},{"$f":"F49-F48","v":{"$error":"#DIV/0!"}},"Should be \nbetween \n3.1 and 3.6"]
[null,null,3,0.2,-0.69897000433601875,{"$f":"H20","v":{"$error":"#DIV/0!"}},{"$f":"F50-F49","v":{"$error":"#DIV/0!"}}]
[null,null,4,0.02,-1.6989700043360187,{"$f":"H23","v":{"$error":"#DIV/0!"}},{"$f":"F51-F50","v":{"$error":"#DIV/0!"}}]
[null,null,5,2E-3,-2.6989700043360187,{"$f":"H26","v":{"$error":"#DIV/0!"}},{"$f":"F52-F51","v":{"$error":"#DIV/0!"}}]
[null,null,6,2.0000000000000001E-4,-3.6989700043360187,{"$f":"H29","v":{"$error":"#DIV/0!"}},{"$f":"F53-F52","v":{"$error":"#DIV/0!"}}]
[null,null,"Efficiency:",{"$f":"POWER(10, 1/(-SLOPE(F48:F53, LOG(D48:D53))))-1","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 90 and 110%"]
[null,null,"Slope:",{"$f":"SLOPE(F48:F53, LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Value must be the same as on the graph"]
[null,null,"R-squared:",{"$f":"RSQ(F48:F53, -LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 0.99 and 1.00"]
[null,null,"Intercept:",11.217000000000001,"(Type the intercept value from the graph in cell D57)"]
[]
[null,null,"If slope =",-3.2393999999999998,"(Type the slope value from the graph in cell D59)"]
[null,null,"then efficiency =",{"$f":"POWER(10, (-1/D59))-1","v":1.0356314022911079},"(Calculated)","Value must be the same as in cell D54"]
[]
[]
[null,null,"Note: it is important to type the appropriate values from the straight line equation into the two green blocks,"]
[null,null,"as the table in Section 3 uses these two values to calculate the concentration of the library samples."]
[]
[]
[]
[]
[]
[null,null,"Section 3. Calculate and review library concentrations"]
[]
[null,null,"- Sort the data for your library samples by grouping the Cq values for different dilutions of the same sample together. Enter the appropriate information into the fields highlighted in green (Columns C - G). "]
[null,null,"- Move the outliers  to Column H, so these are no longer is used in calculations. If you move a Cq value (outlier) from column F to H, you have to delete the formula in column J of that row."]
[]
[null,null,"If the average Cq value for a library < than the average Cq value for Std 1, or > than the average Cq value for Std 6, the data from that dilution may not be used in calculations (i.e. you may not extrapolate). If only one dilution of each library was assayed, the library has to"]
[null,null,"requantified using a more appropriate dilution."]
[]
[]
[null,null,"Library #","Sample name","Dilution","Cq","Average fragment length (bp)","Outliers/\noutside curve","Average Cq","Difference","log\n(concentration)","Average concentration (pM)","Size-adjusted concentration (pM)","Concentration  of undiluted library (pM)","Concentration  of undiluted library (nM)","Concentration of undiluted library (ng/µL)"]
[null,null,1,null,null,null,null,null,{"$f":"AVERAGE(F80:F82)","v":{"$error":"#DIV/0!"}},{"$f":"F80-I80","v":{"$error":"#DIV/0!"}},{"$f":"(I80-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K80","v":{"$error":"#DIV/0!"}},{"$f":"L80*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"M80*E80","v":{"$error":"#DIV/0!"}},{"$f":"N80/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O80*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F81-I80","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F82-I80","v":{"$error":"#DIV/0!"}}]
[null,null,2,null,null,null,null,null,{"$f":"AVERAGE(F83:F85)","v":{"$error":"#DIV/0!"}},{"$f":"F83-I83","v":{"$error":"#DIV/0!"}},{"$f":"(I83-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K83","v":{"$error":"#DIV/0!"}},{"$f":"L83*(452/G83)","v":{"$error":"#DIV/0!"}},{"$f":"M83*E83","v":{"$error":"#DIV/0!"}},{"$f":"N83/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O83*10^-12)*(G83*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F84-I83","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F85-I83","v":{"$error":"#DIV/0!"}}]
[null,null,3,null,null,null,null,null,{"$f":"AVERAGE(F86:F88)","v":{"$error":"#DIV/0!"}},{"$f":"F86-I86","v":{"$error":"#DIV/0!"}},{"$f":"(I86-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K86","v":{"$error":"#DIV/0!"}},{"$f":"L86*(452/G86)","v":{"$error":"#DIV/0!"}},{"$f":"M86*E86","v":{"$error":"#DIV/0!"}},{"$f":"N86/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O86*10^-12)*(G86*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F87-I86","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F88-I86","v":{"$error":"#DIV/0!"}}]
[null,null,4,null,null,null,null,null,{"$f":"AVERAGE(F89:F91)","v":{"$error":"#DIV/0!"}},{"$f":"F89-I89","v":{"$error":"#DIV/0!"}},{"$f":"(I89-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K89","v":{"$error":"#DIV/0!"}},{"$f":"L89*(452/G89)","v":{"$error":"#DIV/0!"}},{"$f":"M89*E89","v":{"$error":"#DIV/0!"}},{"$f":"N89/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O89*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F90-I89","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F91-I89","v":{"$error":"#DIV/0!"}}]
[null,null,5,null,null,null,null,null,{"$f":"AVERAGE(F92:F94)","v":{"$error":"#DIV/0!"}},{"$f":"F92-I92","v":{"$error":"#DIV/0!"}},{"$f":"(I92-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K92","v":{"$error":"#DIV/0!"}},{"$f":"L92*(452/G92)","v":{"$error":"#DIV/0!"}},{"$f":"M92*E92","v":{"$error":"#DIV/0!"}},{"$f":"N92/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O92*10^-12)*(G92*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F93-I92","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F94-I92","v":{"$error":"#DIV/0!"}}]
[null,null,6,null,null,null,null,null,{"$f":"AVERAGE(F95:F97)","v":{"$error":"#DIV/0!"}},{"$f":"F95-I95","v":{"$error":"#DIV/0!"}},{"$f":"(I95-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K95","v":{"$error":"#DIV/0!"}},{"$f":"L95*(452/G95)","v":{"$error":"#DIV/0!"}},{"$f":"M95*E95","v":{"$error":"#DIV/0!"}},{"$f":"N95/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O95*10^-12)*(G95*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F96-I95","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F97-I95","v":{"$error":"#DIV/0!"}}]
[null,null,7,null,null,null,null,null,{"$f":"AVERAGE(F98:F100)","v":{"$error":"#DIV/0!"}},{"$f":"F98-I98","v":{"$error":"#DIV/0!"}},{"$f":"(I98-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K98","v":{"$error":"#DIV/0!"}},{"$f":"L98*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"M98*E98","v":{"$error":"#DIV/0!"}},{"$f":"N98/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O98*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F99-I98","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F100-I98","v":{"$error":"#DIV/0!"}}]
[null,null,8,null,null,null,null,null,{"$f":"AVERAGE(F101:F103)","v":{"$error":"#DIV/0!"}},{"$f":"F101-I101","v":{"$error":"#DIV/0!"}},{"$f":"(I101-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K101","v":{"$error":"#DIV/0!"}},{"$f":"L101*(452/G101)","v":{"$error":"#DIV/0!"}},{"$f":"M101*E101","v":{"$error":"#DIV/0!"}},{"$f":"N101/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O101*10^-12)*(G101*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F102-I101","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F103-I101","v":{"$error":"#DIV/0!"}}]
[null,null,9,null,null,null,null,null,{"$f":"AVERAGE(F104:F106)","v":{"$error":"#DIV/0!"}},{"$f":"F104-I104","v":{"$error":"#DIV/0!"}},{"$f":"(I104-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K104","v":{"$error":"#DIV/0!"}},{"$f":"L104*(452/G104)","v":{"$error":"#DIV/0!"}},{"$f":"M104*E104","v":{"$error":"#DIV/0!"}},{"$f":"N104/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O104*10^-12)*(G104*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F105-I104","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F106-I104","v":{"$error":"#DIV/0!"}}]
[null,null,10,null,null,null,null,null,{"$f":"AVERAGE(F107:F109)","v":{"$error":"#DIV/0!"}},{"$f":"F107-I107","v":{"$error":"#DIV/0!"}},{"$f":"(I107-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K107","v":{"$error":"#DIV/0!"}},{"$f":"L107*(452/G107)","v":{"$error":"#DIV/0!"}},{"$f":"M107*E107","v":{"$error":"#DIV/0!"}},{"$f":"N107/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O107*10^-12)*(G107*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F108-I107","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F109-I107","v":{"$error":"#DIV/0!"}}]
[null,null,11,null,null,null,null,null,{"$f":"AVERAGE(F110:F112)","v":{"$error":"#DIV/0!"}},{"$f":"F110-I110","v":{"$error":"#DIV/0!"}},{"$f":"(I110-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K110","v":{"$error":"#DIV/0!"}},{"$f":"L110*(452/G110)","v":{"$error":"#DIV/0!"}},{"$f":"M110*E110","v":{"$error":"#DIV/0!"}},{"$f":"N110/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O110*10^-12)*(G110*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F111-I110","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F112-I110","v":{"$error":"#DIV/0!"}}]
[null,null,12,null,null,null,null,null,{"$f":"AVERAGE(F113:F115)","v":{"$error":"#DIV/0!"}},{"$f":"F113-I113","v":{"$error":"#DIV/0!"}},{"$f":"(I113-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K113","v":{"$error":"#DIV/0!"}},{"$f":"L113*(452/G113)","v":{"$error":"#DIV/0!"}},{"$f":"M113*E113","v":{"$error":"#DIV/0!"}},{"$f":"N113/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O113*10^-12)*(G113*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F114-I113","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F115-I113","v":{"$error":"#DIV/0!"}}]
[null,null,13,null,null,null,null,null,{"$f":"AVERAGE(F116:F118)","v":{"$error":"#DIV/0!"}},{"$f":"F116-I116","v":{"$error":"#DIV/0!"}},{"$f":"(I116-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K116","v":{"$error":"#DIV/0!"}},{"$f":"L116*(452/G116)","v":{"$error":"#DIV/0!"}},{"$f":"M116*E116","v":{"$error":"#DIV/0!"}},{"$f":"N116/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O116*10^-12)*(G116*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F117-I116","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F118-I116","v":{"$error":"#DIV/0!"}}]
[null,null,14,null,null,null,null,null,{"$f":"AVERAGE(F119:F121)","v":{"$error":"#DIV/0!"}},{"$f":"F119-I119","v":{"$error":"#DIV/0!"}},{"$f":"(I119-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K119","v":{"$error":"#DIV/0!"}},{"$f":"L119*(452/G119)","v":{"$error":"#DIV/0!"}},{"$f":"M119*E119","v":{"$error":"#DIV/0!"}},{"$f":"N119/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O119*10^-12)*(G119*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F120-I119","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F121-I119","v":{"$error":"#DIV/0!"}}]
[null,null,15,null,null,null,null,null,{"$f":"AVERAGE(F122:F124)","v":{"$error":"#DIV/0!"}},{"$f":"F122-I122","v":{"$error":"#DIV/0!"}},{"$f":"(I122-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K122","v":{"$error":"#DIV/0!"}},{"$f":"L122*(452/G122)","v":{"$error":"#DIV/0!"}},{"$f":"M122*E122","v":{"$error":"#DIV/0!"}},{"$f":"N122/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O122*10^-12)*(G122*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F123-I122","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F124-I122","v":{"$error":"#DIV/0!"}}]
[null,null,16,null,null,null,null,null,{"$f":"AVERAGE(F125:F127)","v":{"$error":"#DIV/0!"}},{"$f":"F125-I125","v":{"$error":"#DIV/0!"}},{"$f":"(I125-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K125","v":{"$error":"#DIV/0!"}},{"$f":"L125*(452/G125)","v":{"$error":"#DIV/0!"}},{"$f":"M125*E125","v":{"$error":"#DIV/0!"}},{"$f":"N125/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O125*10^-12)*(G125*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F126-I125","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F127-I125","v":{"$error":"#DIV/0!"}}]
[null,null,17,null,null,null,null,null,{"$f":"AVERAGE(F128:F130)","v":{"$error":"#DIV/0!"}},{"$f":"F128-I128","v":{"$error":"#DIV/0!"}},{"$f":"(I128-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^K128","v":{"$error":"#DIV/0!"}},{"$f":"L128*(452/G128)","v":{"$error":"#DIV/0!"}},{"$f":"M128*E128","v":{"$error":"#DIV/0!"}},{"$f":"N128/1000","v":{"$error":"#DIV/0!"}},{"$f":"((O128*10^-12)*(G128*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F129-I128","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F130-I128","v":{"$error":"#DIV/0!"}}]
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{"sheet":"1 dilution - Summary","index":2,"n_rows":101,"n_cols":8,"rows_kept":101,"cut":false,"hidden":true,"merged":[]}
[]
[]
[null,null,"Library concentrations and yields"]
[]
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[]
[]
[null,null,"Section 1. Review Cq values for DNA Standards"]
[]
[null,null,"- Enter the appropriate information into the fields highlighted in green."]
[null,null,"- Move  \"outliers\" to column G (so these are no longer is used in calculations). Delete the formula in the corresponding row in column I."]
[null,null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between 3.2 and 3.45 is very good  "]
[null,null,"   and 3.1 - 3.6 is acceptable)."]
[null,null,"- If the spacing between any two standards is less than 3.1 cycles and more than 3.6 cycles, those data points (and any library samples falling between those"]
[null,null,"  data points) are not highly reliable."]
[]
[]
[null,null,"Well","Std #","Conc (pM)","Cq","Outliers","Av Cq","Difference","Delta Cq"]
[null,null,null,1,20,null,null,{"$f":"AVERAGE(F14:F16)","v":{"$error":"#DIV/0!"}},{"$f":"F14-$H$14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,null,1,20,null,null,null,{"$f":"F15-$H$14","v":{"$error":"#DIV/0!"}}]
[null,null,null,1,20,null,null,null,{"$f":"F16-$H$14","v":{"$error":"#DIV/0!"}},{"$f":"H17-H14","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,{"$f":"AVERAGE(F17:F19)","v":{"$error":"#DIV/0!"}},{"$f":"F17-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F18-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F19-$H$17","v":{"$error":"#DIV/0!"}},{"$f":"H20-H17","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,{"$f":"AVERAGE(F20:F22)","v":{"$error":"#DIV/0!"}},{"$f":"F20-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F21-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F22-$H$20","v":{"$error":"#DIV/0!"}},{"$f":"H23-H20","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,{"$f":"AVERAGE(F23:F25)","v":{"$error":"#DIV/0!"}},{"$f":"F23-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F24-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F25-$H$23","v":{"$error":"#DIV/0!"}},{"$f":"H26-H23","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,{"$f":"AVERAGE(F26:F28)","v":{"$error":"#DIV/0!"}},{"$f":"F26-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F27-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F28-$H$26","v":{"$error":"#DIV/0!"}},{"$f":"H29-H26","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,{"$f":"AVERAGE(F29:F31)","v":{"$error":"#DIV/0!"}},{"$f":"F29-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F30-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F31-$H$29","v":{"$error":"#DIV/0!"}},{"$f":"H32-H29","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-",null,null,{"$f":"AVERAGE(F32:F34)","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-"]
[null,null,null,"NTC","-"]
[]
[]
[]
[]
[]
[null,null,"Section 2. Generate and review the standard curve"]
[]
[null,null,"- Type the value for the intercept from the graph to the right into cell D57."]
[null,null,"- Type the value for the slope from the graph to the right into cell D59. ",null,null,null,null,null,null,null,"Note: if the standard curve equation does not update, click on the line, right click and select"]
[null,null,null,null,null,null,null,null,null,null,"Format Trendline. (Untick and) tick the boxes for \"Display Equation and R-squared value on chart\""]
[]
[]
[null,null,"DNA Standard","Conc in pM","Log conc","Average Cq","Delta Cq"]
[null,null,1,20,{"$f":"LOG(D48)","v":1.3010299956639813},{"$f":"H14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,2,2,0.3010299956639812,{"$f":"H17","v":{"$error":"#DIV/0!"}},{"$f":"F49-F48","v":{"$error":"#DIV/0!"}},"Should be \nbetween \n3.1 and 3.6"]
[null,null,3,0.2,-0.69897000433601875,{"$f":"H20","v":{"$error":"#DIV/0!"}},{"$f":"F50-F49","v":{"$error":"#DIV/0!"}}]
[null,null,4,0.02,-1.6989700043360187,{"$f":"H23","v":{"$error":"#DIV/0!"}},{"$f":"F51-F50","v":{"$error":"#DIV/0!"}}]
[null,null,5,2E-3,-2.6989700043360187,{"$f":"H26","v":{"$error":"#DIV/0!"}},{"$f":"F52-F51","v":{"$error":"#DIV/0!"}}]
[null,null,6,2.0000000000000001E-4,-3.6989700043360187,{"$f":"H29","v":{"$error":"#DIV/0!"}},{"$f":"F53-F52","v":{"$error":"#DIV/0!"}}]
[null,null,"Efficiency:",{"$f":"POWER(10, 1/(-SLOPE(F48:F53, LOG(D48:D53))))-1","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 90 and 110%"]
[null,null,"Slope:",{"$f":"SLOPE(F48:F53, LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Value must be the same as on the graph"]
[null,null,"R-squared:",{"$f":"RSQ(F48:F53, -LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 0.99 and 1.00"]
[null,null,"Intercept:",11.217000000000001,"(Type the intercept value from the graph in cell D57)"]
[]
[null,null,"If slope =",-3.2393999999999998,"(Type the slope value from the graph in cell D59)"]
[null,null,"then efficiency =",{"$f":"POWER(10, (-1/D59))-1","v":1.0356314022911079},"(Calculated)","Value must be the same as in cell D54"]
[]
[]
[null,null,"Note: it is important to type the appropriate values from the straight line equation into the two green blocks,"]
[null,null,"as the table in Section 3 uses these two values to calculate the concentration of the library samples."]
[]
[]
[]
[]
[]
[null,null,"Section 3. Calculate and review library concentrations"]
[]
[null,null,"- Sort the data for your library samples by grouping the Cq values for different dilutions of the same sample together. Enter the appropriate information into the fields highlighted in green (Columns C - G). "]
[null,null,"- Move the outliers  to Column H, so these are no longer is used in calculations. If you move a Cq value (outlier) from column F to H, you have to delete the formula in column J of that row."]
[]
[null,null,"If the average Cq value for a library < than the average Cq value for Std 1, or > than the average Cq value for Std 6, the data from that dilution may not be used in calculations (i.e. you may not extrapolate). If only one dilution of each library was assayed, the library has to"]
[null,null,"requantified using a more appropriate dilution."]
[]
[]
[null,null,"Library #","Sample name","Dilution","Cq","Average fragment length (bp)","Outliers/\noutside curve","Average Cq","Difference","Delta Cq","log\n(concentration)","Average concentration (pM)","Size-adjusted concentration (pM)","Concentration  of undiluted library (pM)","Concentration  of undiluted library (nM)","Concentration of undiluted library (ng/µL)","% Deviation","Working concentration (pM)","Working concentration (nM)","Working concentration (ng/µL)"]
[null,null,1,null,null,null,null,null,{"$f":"AVERAGE(F80:F82)","v":{"$error":"#DIV/0!"}},{"$f":"F80-I80","v":{"$error":"#DIV/0!"}},{"$f":"I83-I80","v":{"$error":"#DIV/0!"}},{"$f":"(I80-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L80","v":{"$error":"#DIV/0!"}},{"$f":"M80*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"N80*E80","v":{"$error":"#DIV/0!"}},{"$f":"O80/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P80*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P83/P80)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O80:O85)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P80:P85)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q80:Q85)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F81-I80","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F82-I80","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F83:F85)","v":{"$error":"#DIV/0!"}},{"$f":"F83-I83","v":{"$error":"#DIV/0!"}},null,{"$f":"(I83-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L83","v":{"$error":"#DIV/0!"}},{"$f":"M83*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"N83*E83","v":{"$error":"#DIV/0!"}},{"$f":"O83/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P83*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F84-I83","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F85-I83","v":{"$error":"#DIV/0!"}}]
[null,null,2,null,null,null,null,"`",{"$f":"AVERAGE(F86:F88)","v":{"$error":"#DIV/0!"}},{"$f":"F86-I86","v":{"$error":"#DIV/0!"}},{"$f":"I89-I86","v":{"$error":"#DIV/0!"}},{"$f":"(I86-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L86","v":{"$error":"#DIV/0!"}},{"$f":"M86*(452/G86)","v":{"$error":"#DIV/0!"}},{"$f":"N86*E86","v":{"$error":"#DIV/0!"}},{"$f":"O86/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P86*10^-12)*(G86*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P89/P86)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O86:O91)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P86:P91)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q86:Q91)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F87-I86","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F88-I86","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F89:F91)","v":{"$error":"#DIV/0!"}},{"$f":"F89-I89","v":{"$error":"#DIV/0!"}},null,{"$f":"(I89-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L89","v":{"$error":"#DIV/0!"}},{"$f":"M89*(452/G86)","v":{"$error":"#DIV/0!"}},{"$f":"N89*E89","v":{"$error":"#DIV/0!"}},{"$f":"O89/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P89*10^-12)*(G86*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F90-I89","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F91-I89","v":{"$error":"#DIV/0!"}}]
[null,null,3,null,null,null,null,null,{"$f":"AVERAGE(F92:F94)","v":{"$error":"#DIV/0!"}},{"$f":"F92-I92","v":{"$error":"#DIV/0!"}},{"$f":"I95-I92","v":{"$error":"#DIV/0!"}},{"$f":"(I92-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L92","v":{"$error":"#DIV/0!"}},{"$f":"M92*(452/G92)","v":{"$error":"#DIV/0!"}},{"$f":"N92*E92","v":{"$error":"#DIV/0!"}},{"$f":"O92/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P92*10^-12)*(G92*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P95/P92)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O92:O97)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P92:P97)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q92:Q97)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F93-I92","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F94-I92","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F95:F97)","v":{"$error":"#DIV/0!"}},{"$f":"F95-I95","v":{"$error":"#DIV/0!"}},null,{"$f":"(I95-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L95","v":{"$error":"#DIV/0!"}},{"$f":"M95*(452/G92)","v":{"$error":"#DIV/0!"}},{"$f":"N95*E95","v":{"$error":"#DIV/0!"}},{"$f":"O95/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P95*10^-12)*(G92*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F96-I95","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F97-I95","v":{"$error":"#DIV/0!"}}]
[null,null,4,null,null,null,null,null,{"$f":"AVERAGE(F98:F100)","v":{"$error":"#DIV/0!"}},{"$f":"F98-I98","v":{"$error":"#DIV/0!"}},{"$f":"I101-I98","v":{"$error":"#DIV/0!"}},{"$f":"(I98-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L98","v":{"$error":"#DIV/0!"}},{"$f":"M98*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"N98*E98","v":{"$error":"#DIV/0!"}},{"$f":"O98/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P98*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P101/P98)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O98:O103)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P98:P103)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q98:Q103)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F99-I98","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F100-I98","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F101:F103)","v":{"$error":"#DIV/0!"}},{"$f":"F101-I101","v":{"$error":"#DIV/0!"}},null,{"$f":"(I101-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L101","v":{"$error":"#DIV/0!"}},{"$f":"M101*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"N101*E101","v":{"$error":"#DIV/0!"}},{"$f":"O101/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P101*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F102-I101","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F103-I101","v":{"$error":"#DIV/0!"}}]
[null,null,5,null,null,null,null,null,{"$f":"AVERAGE(F104:F106)","v":{"$error":"#DIV/0!"}},{"$f":"F104-I104","v":{"$error":"#DIV/0!"}},{"$f":"I107-I104","v":{"$error":"#DIV/0!"}},{"$f":"(I104-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L104","v":{"$error":"#DIV/0!"}},{"$f":"M104*(452/G104)","v":{"$error":"#DIV/0!"}},{"$f":"N104*E104","v":{"$error":"#DIV/0!"}},{"$f":"O104/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P104*10^-12)*(G104*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P107/P104)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O104:O109)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P104:P109)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q104:Q109)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F105-I104","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F106-I104","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F107:F109)","v":{"$error":"#DIV/0!"}},{"$f":"F107-I107","v":{"$error":"#DIV/0!"}},null,{"$f":"(I107-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L107","v":{"$error":"#DIV/0!"}},{"$f":"M107*(452/G104)","v":{"$error":"#DIV/0!"}},{"$f":"N107*E107","v":{"$error":"#DIV/0!"}},{"$f":"O107/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P107*10^-12)*(G104*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F108-I107","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F109-I107","v":{"$error":"#DIV/0!"}}]
[null,null,6,null,null,null,null,null,{"$f":"AVERAGE(F110:F112)","v":{"$error":"#DIV/0!"}},{"$f":"F110-I110","v":{"$error":"#DIV/0!"}},{"$f":"I113-I110","v":{"$error":"#DIV/0!"}},{"$f":"(I110-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L110","v":{"$error":"#DIV/0!"}},{"$f":"M110*(452/G110)","v":{"$error":"#DIV/0!"}},{"$f":"N110*E110","v":{"$error":"#DIV/0!"}},{"$f":"O110/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P110*10^-12)*(G110*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P113/P110)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O110:O115)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P110:P115)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q110:Q115)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F111-I110","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F112-I110","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F113:F115)","v":{"$error":"#DIV/0!"}},{"$f":"F113-I113","v":{"$error":"#DIV/0!"}},null,{"$f":"(I113-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L113","v":{"$error":"#DIV/0!"}},{"$f":"M113*(452/G110)","v":{"$error":"#DIV/0!"}},{"$f":"N113*E113","v":{"$error":"#DIV/0!"}},{"$f":"O113/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P113*10^-12)*(G110*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F114-I113","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F115-I113","v":{"$error":"#DIV/0!"}}]
[null,null,7,null,null,null,null,null,{"$f":"AVERAGE(F116:F118)","v":{"$error":"#DIV/0!"}},{"$f":"F116-I116","v":{"$error":"#DIV/0!"}},{"$f":"I119-I116","v":{"$error":"#DIV/0!"}},{"$f":"(I116-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L116","v":{"$error":"#DIV/0!"}},{"$f":"M116*(452/G116)","v":{"$error":"#DIV/0!"}},{"$f":"N116*E116","v":{"$error":"#DIV/0!"}},{"$f":"O116/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P116*10^-12)*(G116*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P119/P116)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(O116:O121)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P116:P121)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q116:Q121)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F117-I116","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F118-I116","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F119:F121)","v":{"$error":"#DIV/0!"}},{"$f":"F119-I119","v":{"$error":"#DIV/0!"}},null,{"$f":"(I119-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L119","v":{"$error":"#DIV/0!"}},{"$f":"M119*(452/G116)","v":{"$error":"#DIV/0!"}},{"$f":"N119*E119","v":{"$error":"#DIV/0!"}},{"$f":"O119/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P119*10^-12)*(G116*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}}]
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{"sheet":"2 dilutions - Summary","index":4,"n_rows":53,"n_cols":8,"rows_kept":53,"cut":false,"hidden":true,"merged":[]}
[]
[]
[null,null,"Library concentrations and yields"]
[]
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[]
[]
[null,null,"Section 1. Review Cq values for DNA Standards"]
[]
[null,null,"- Enter the appropriate information into the fields highlighted in green."]
[null,null,"- Move  \"outliers\" to column G (so these are no longer is used in calculations). Delete the formula in the corresponding row in column I."]
[null,null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between 3.2 and 3.45 is very good  "]
[null,null,"   and 3.1 - 3.6 is acceptable)."]
[null,null,"- If the spacing between any two standards is less than 3.1 cycles and more than 3.6 cycles, those data points (and any library samples falling between those"]
[null,null,"  data points) are not highly reliable."]
[]
[]
[null,null,"Well","Std #","Conc (pM)","Cq","Outliers","Av Cq","Difference","Delta Cq"]
[null,null,null,1,20,null,null,{"$f":"AVERAGE(F14:F16)","v":{"$error":"#DIV/0!"}},{"$f":"F14-$H$14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,null,1,20,null,null,null,{"$f":"F15-$H$14","v":{"$error":"#DIV/0!"}}]
[null,null,null,1,20,null,null,null,{"$f":"F16-$H$14","v":{"$error":"#DIV/0!"}},{"$f":"H17-H14","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,{"$f":"AVERAGE(F17:F19)","v":{"$error":"#DIV/0!"}},{"$f":"F17-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F18-$H$17","v":{"$error":"#DIV/0!"}}]
[null,null,null,2,2,null,null,null,{"$f":"F19-$H$17","v":{"$error":"#DIV/0!"}},{"$f":"H20-H17","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,{"$f":"AVERAGE(F20:F22)","v":{"$error":"#DIV/0!"}},{"$f":"F20-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F21-$H$20","v":{"$error":"#DIV/0!"}}]
[null,null,null,3,0.2,null,null,null,{"$f":"F22-$H$20","v":{"$error":"#DIV/0!"}},{"$f":"H23-H20","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,{"$f":"AVERAGE(F23:F25)","v":{"$error":"#DIV/0!"}},{"$f":"F23-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F24-$H$23","v":{"$error":"#DIV/0!"}}]
[null,null,null,4,0.02,null,null,null,{"$f":"F25-$H$23","v":{"$error":"#DIV/0!"}},{"$f":"H26-H23","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,{"$f":"AVERAGE(F26:F28)","v":{"$error":"#DIV/0!"}},{"$f":"F26-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F27-$H$26","v":{"$error":"#DIV/0!"}}]
[null,null,null,5,2E-3,null,null,null,{"$f":"F28-$H$26","v":{"$error":"#DIV/0!"}},{"$f":"H29-H26","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,{"$f":"AVERAGE(F29:F31)","v":{"$error":"#DIV/0!"}},{"$f":"F29-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F30-$H$29","v":{"$error":"#DIV/0!"}}]
[null,null,null,6,2.0000000000000001E-4,null,null,null,{"$f":"F31-$H$29","v":{"$error":"#DIV/0!"}},{"$f":"H32-H29","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-",null,null,{"$f":"AVERAGE(F32:F34)","v":{"$error":"#DIV/0!"}}]
[null,null,null,"NTC","-"]
[null,null,null,"NTC","-"]
[]
[]
[]
[]
[]
[null,null,"Section 2. Generate and review the standard curve"]
[]
[null,null,"- Type the value for the intercept from the graph to the right into cell D57."]
[null,null,"- Type the value for the slope from the graph to the right into cell D59. ",null,null,null,null,null,null,null,"Note: if the standard curve equation does not update, click on the line, right click and select"]
[null,null,null,null,null,null,null,null,null,null,"Format Trendline. (Untick and) tick the boxes for \"Display Equation and R-squared value on chart\""]
[]
[]
[null,null,"DNA Standard","Conc in pM","Log conc","Average Cq","Delta Cq"]
[null,null,1,20,{"$f":"LOG(D48)","v":1.3010299956639813},{"$f":"H14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,2,2,0.3010299956639812,{"$f":"H17","v":{"$error":"#DIV/0!"}},{"$f":"F49-F48","v":{"$error":"#DIV/0!"}},"Should be \nbetween \n3.1 and 3.6"]
[null,null,3,0.2,-0.69897000433601875,{"$f":"H20","v":{"$error":"#DIV/0!"}},{"$f":"F50-F49","v":{"$error":"#DIV/0!"}}]
[null,null,4,0.02,-1.6989700043360187,{"$f":"H23","v":{"$error":"#DIV/0!"}},{"$f":"F51-F50","v":{"$error":"#DIV/0!"}}]
[null,null,5,2E-3,-2.6989700043360187,{"$f":"H26","v":{"$error":"#DIV/0!"}},{"$f":"F52-F51","v":{"$error":"#DIV/0!"}}]
[null,null,6,2.0000000000000001E-4,-3.6989700043360187,{"$f":"H29","v":{"$error":"#DIV/0!"}},{"$f":"F53-F52","v":{"$error":"#DIV/0!"}}]
[null,null,"Efficiency:",{"$f":"POWER(10, 1/(-SLOPE(F48:F53, LOG(D48:D53))))-1","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 90 and 110%"]
[null,null,"Slope:",{"$f":"SLOPE(F48:F53, LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Value must be the same as on the graph"]
[null,null,"R-squared:",{"$f":"RSQ(F48:F53, -LOG(D48:D53))","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 0.99 and 1.00"]
[null,null,"Intercept:",11.217000000000001,"(Type the intercept value from the graph in cell D57)"]
[]
[null,null,"If slope =",-3.2393999999999998,"(Type the slope value from the graph in cell D59)"]
[null,null,"then efficiency =",{"$f":"POWER(10, (-1/D59))-1","v":1.0356314022911079},"(Calculated)","Value must be the same as in cell D54"]
[]
[]
[null,null,"Note: it is important to type the appropriate values from the straight line equation into the two green blocks,"]
[null,null,"as the table in Section 3 uses these two values to calculate the concentration of the library samples."]
[]
[]
[]
[]
[]
[null,null,"Section 3. Calculate and review library concentrations"]
[]
[null,null,"- Sort the data for your library samples by grouping the Cq values for different dilutions of the same sample together. Enter the appropriate information into the fields highlighted in green (Columns C - G). "]
[null,null,"- Move the outliers  to Column H, so these are no longer is used in calculations. If you move a Cq value (outlier) from column F to H, you have to delete the formula in column J of that row."]
[]
[null,null,"If the average Cq value for a library < than the average Cq value for Std 1, or > than the average Cq value for Std 6, the data from that dilution may not be used in calculations (i.e. you may not extrapolate). If only one dilution of each library was assayed, the library has to"]
[null,null,"requantified using a more appropriate dilution."]
[]
[]
[null,null,"Library #","Sample name","Dilution","Cq","Average fragment length (bp)","Outliers/\noutside curve","Average Cq","Difference","Delta Cq","log\n(concentration)","Average concentration (pM)","Size-adjusted concentration (pM)","Concentration  of undiluted library (pM)","Concentration  of undiluted library (nM)","Concentration of undiluted library (ng/µL)","% Deviation","Working concentration (pM)","Working concentration (nM)","Working concentration (ng/µL)"]
[null,null,1,null,null,null,null,null,{"$f":"AVERAGE(F80:F82)","v":{"$error":"#DIV/0!"}},{"$f":"F80-I80","v":{"$error":"#DIV/0!"}},"-",{"$f":"(I80-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L80","v":{"$error":"#DIV/0!"}},{"$f":"M80*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"N80*E80","v":{"$error":"#DIV/0!"}},{"$f":"O80/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P80*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O80:O88)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P80:P88)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q80:Q88)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F81-I80","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F82-I80","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F83:F85)","v":{"$error":"#DIV/0!"}},{"$f":"F83-I83","v":{"$error":"#DIV/0!"}},{"$f":"I83-I80","v":{"$error":"#DIV/0!"}},{"$f":"(I83-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L83","v":{"$error":"#DIV/0!"}},{"$f":"M83*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"N83*E83","v":{"$error":"#DIV/0!"}},{"$f":"O83/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P83*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P83/P80)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F84-I83","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F85-I83","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,"`",{"$f":"AVERAGE(F86:F88)","v":{"$error":"#DIV/0!"}},{"$f":"F86-I86","v":{"$error":"#DIV/0!"}},{"$f":"I86-I83","v":{"$error":"#DIV/0!"}},{"$f":"(I86-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L86","v":{"$error":"#DIV/0!"}},{"$f":"M86*(452/G80)","v":{"$error":"#DIV/0!"}},{"$f":"N86*E86","v":{"$error":"#DIV/0!"}},{"$f":"O86/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P86*10^-12)*(G80*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P86/P80)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F87-I86","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F88-I86","v":{"$error":"#DIV/0!"}}]
[null,null,2,null,null,null,null,null,{"$f":"AVERAGE(F89:F91)","v":{"$error":"#DIV/0!"}},{"$f":"F89-I89","v":{"$error":"#DIV/0!"}},"-",{"$f":"(I89-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L89","v":{"$error":"#DIV/0!"}},{"$f":"M89*(452/G89)","v":{"$error":"#DIV/0!"}},{"$f":"N89*E89","v":{"$error":"#DIV/0!"}},{"$f":"O89/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P89*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O89:O97)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P89:P97)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q89:Q97)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F90-I89","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F91-I89","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F92:F94)","v":{"$error":"#DIV/0!"}},{"$f":"F92-I92","v":{"$error":"#DIV/0!"}},{"$f":"I92-I89","v":{"$error":"#DIV/0!"}},{"$f":"(I92-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L92","v":{"$error":"#DIV/0!"}},{"$f":"M92*(452/G89)","v":{"$error":"#DIV/0!"}},{"$f":"N92*E92","v":{"$error":"#DIV/0!"}},{"$f":"O92/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P92*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P92/P89)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F93-I92","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F94-I92","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F95:F97)","v":{"$error":"#DIV/0!"}},{"$f":"F95-I95","v":{"$error":"#DIV/0!"}},{"$f":"I95-I92","v":{"$error":"#DIV/0!"}},{"$f":"(I95-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L95","v":{"$error":"#DIV/0!"}},{"$f":"M95*(452/G89)","v":{"$error":"#DIV/0!"}},{"$f":"N95*E95","v":{"$error":"#DIV/0!"}},{"$f":"O95/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P95*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P95/P89)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F96-I95","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F97-I95","v":{"$error":"#DIV/0!"}}]
[null,null,3,null,null,null,null,null,{"$f":"AVERAGE(F98:F100)","v":{"$error":"#DIV/0!"}},{"$f":"F98-I98","v":{"$error":"#DIV/0!"}},"-",{"$f":"(I98-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L98","v":{"$error":"#DIV/0!"}},{"$f":"M98*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"N98*E98","v":{"$error":"#DIV/0!"}},{"$f":"O98/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P98*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O98:O106)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P98:P106)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q98:Q106)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F99-I98","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F100-I98","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F101:F103)","v":{"$error":"#DIV/0!"}},{"$f":"F101-I101","v":{"$error":"#DIV/0!"}},{"$f":"I101-I98","v":{"$error":"#DIV/0!"}},{"$f":"(I101-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L101","v":{"$error":"#DIV/0!"}},{"$f":"M101*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"N101*E101","v":{"$error":"#DIV/0!"}},{"$f":"O101/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P101*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P101/P98)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F102-I101","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F103-I101","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F104:F106)","v":{"$error":"#DIV/0!"}},{"$f":"F104-I104","v":{"$error":"#DIV/0!"}},{"$f":"I104-I101","v":{"$error":"#DIV/0!"}},{"$f":"(I104-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L104","v":{"$error":"#DIV/0!"}},{"$f":"M104*(452/G98)","v":{"$error":"#DIV/0!"}},{"$f":"N104*E104","v":{"$error":"#DIV/0!"}},{"$f":"O104/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P104*10^-12)*(G98*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P104/P98)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F105-I104","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F106-I104","v":{"$error":"#DIV/0!"}}]
[null,null,4,null,null,null,null,null,{"$f":"AVERAGE(F107:F109)","v":{"$error":"#DIV/0!"}},{"$f":"F107-I107","v":{"$error":"#DIV/0!"}},"-",{"$f":"(I107-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L107","v":{"$error":"#DIV/0!"}},{"$f":"M107*(452/G107)","v":{"$error":"#DIV/0!"}},{"$f":"N107*E107","v":{"$error":"#DIV/0!"}},{"$f":"O107/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P107*10^-12)*(G107*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O107:O115)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P107:P115)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q107:Q115)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F108-I107","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F109-I107","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F110:F112)","v":{"$error":"#DIV/0!"}},{"$f":"F110-I110","v":{"$error":"#DIV/0!"}},{"$f":"I110-I107","v":{"$error":"#DIV/0!"}},{"$f":"(I110-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L110","v":{"$error":"#DIV/0!"}},{"$f":"M110*(452/G107)","v":{"$error":"#DIV/0!"}},{"$f":"N110*E110","v":{"$error":"#DIV/0!"}},{"$f":"O110/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P110*10^-12)*(G107*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P110/P107)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F111-I110","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F112-I110","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F113:F115)","v":{"$error":"#DIV/0!"}},{"$f":"F113-I113","v":{"$error":"#DIV/0!"}},{"$f":"I113-I110","v":{"$error":"#DIV/0!"}},{"$f":"(I113-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L113","v":{"$error":"#DIV/0!"}},{"$f":"M113*(452/G107)","v":{"$error":"#DIV/0!"}},{"$f":"N113*E113","v":{"$error":"#DIV/0!"}},{"$f":"O113/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P113*10^-12)*(G107*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P113/P107)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F114-I113","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F115-I113","v":{"$error":"#DIV/0!"}}]
[null,null,5,null,null,null,null,null,{"$f":"AVERAGE(F116:F118)","v":{"$error":"#DIV/0!"}},{"$f":"F116-I116","v":{"$error":"#DIV/0!"}},"-",{"$f":"(I116-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L116","v":{"$error":"#DIV/0!"}},{"$f":"M116*(452/G116)","v":{"$error":"#DIV/0!"}},{"$f":"N116*E116","v":{"$error":"#DIV/0!"}},{"$f":"O116/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P116*10^-12)*(G116*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O116:O124)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P116:P124)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q116:Q124)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F117-I116","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F118-I116","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,{"$f":"AVERAGE(F119:F121)","v":{"$error":"#DIV/0!"}},{"$f":"F119-I119","v":{"$error":"#DIV/0!"}},{"$f":"I119-I116","v":{"$error":"#DIV/0!"}},{"$f":"(I119-$D$57)/$D$59","v":{"$error":"#DIV/0!"}},{"$f":"10^L119","v":{"$error":"#DIV/0!"}},{"$f":"M119*(452/G116)","v":{"$error":"#DIV/0!"}},{"$f":"N119*E119","v":{"$error":"#DIV/0!"}},{"$f":"O119/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P119*10^-12)*(G116*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P119/P116)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F120-I119","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,null,null,null,null,null,{"$f":"F121-I119","v":{"$error":"#DIV/0!"}}]
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{"sheet":"3 dilutions - Summary","index":6,"n_rows":37,"n_cols":8,"rows_kept":37,"cut":false,"hidden":true,"merged":[]}
[]
[]
[null,null,"Library concentrations and yields"]
[]
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[null,null,{"$f":"'3 dilutions - Analysis'!D215","v":0},{"$f":"'3 dilutions - Analysis'!S215","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T215","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U215","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D224","v":0},{"$f":"'3 dilutions - Analysis'!S224","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T224","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U224","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D233","v":0},{"$f":"'3 dilutions - Analysis'!S233","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T233","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U233","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D242","v":0},{"$f":"'3 dilutions - Analysis'!S242","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T242","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U242","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D251","v":0},{"$f":"'3 dilutions - Analysis'!S251","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T251","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U251","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D260","v":0},{"$f":"'3 dilutions - Analysis'!S260","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T260","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U260","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D269","v":0},{"$f":"'3 dilutions - Analysis'!S269","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T269","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U269","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D278","v":0},{"$f":"'3 dilutions - Analysis'!S278","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T278","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U278","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D287","v":0},{"$f":"'3 dilutions - Analysis'!S287","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T287","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U287","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D296","v":0},{"$f":"'3 dilutions - Analysis'!S296","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T296","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U296","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D305","v":0},{"$f":"'3 dilutions - Analysis'!S305","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T305","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U305","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D314","v":0},{"$f":"'3 dilutions - Analysis'!S314","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T314","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U314","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D323","v":0},{"$f":"'3 dilutions - Analysis'!S323","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T323","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U323","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D332","v":0},{"$f":"'3 dilutions - Analysis'!S332","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T332","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U332","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D341","v":0},{"$f":"'3 dilutions - Analysis'!S341","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T341","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U341","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D350","v":0},{"$f":"'3 dilutions - Analysis'!S350","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T350","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U350","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]
[null,null,{"$f":"'3 dilutions - Analysis'!D359","v":0},{"$f":"'3 dilutions - Analysis'!S359","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!T359","v":{"$error":"#DIV/0!"}},{"$f":"'3 dilutions - Analysis'!U359","v":{"$error":"#DIV/0!"}},25,{"$error":"#DIV/0!"}]

{"sheet":"Chromium Connect Assay Info","index":7,"n_rows":12,"n_cols":10,"rows_kept":12,"cut":false,"hidden":false,"merged":[[0,9,1,11]]}
["Fill in the sample names and corresponding average library sizes for this experiment",null,null,null,null,null,"Dilution 1",12500,null,"CG000157 Rev A"]
[null,null,null,null,null,null,"Dilution 2",62500]
[null,null,null,null,null,null,"Dilution 3",312500]
[null,"Sample Name","Average Library Size (bp)",null,null,null,"Dilution 4",1562500]
["Sample 1"]
["Sample 2"]
["Sample 3"]
["Sample 4"]
["Sample 5"]
["Sample 6"]
["Sample 7"]
["Sample 8"]

{"sheet":"Cq data input","index":8,"n_rows":99,"n_cols":10,"rows_kept":99,"cut":false,"hidden":false,"merged":[[0,9,1,11]]}
["Copy and paste the Cq values obtained for this experiment into cells B4-B99",null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
["Well","Cq"]
["A01"]
["A02"]
["A03"]
["A04"]
["A05"]
["A06"]
["A07"]
["A08"]
["A09"]
["A10"]
["A11"]
["A12"]
["B01"]
["B02"]
["B03"]
["B04"]
["B05"]
["B06"]
["B07"]
["B08"]
["B09"]
["B10"]
["B11"]
["B12"]
["C01"]
["C02"]
["C03"]
["C04"]
["C05"]
["C06"]
["C07"]
["C08"]
["C09"]
["C10"]
["C11"]
["C12"]
["D01"]
["D02"]
["D03"]
["D04"]
["D05"]
["D06"]
["D07"]
["D08"]
["D09"]
["D10"]
["D11"]
["D12"]
["E01"]
["E02"]
["E03"]
["E04"]
["E05"]
["E06"]
["E07"]
["E08"]
["E09"]
["E10"]
["E11"]
["E12"]
["F01"]
["F02"]
["F03"]
["F04"]
["F05"]
["F06"]
["F07"]
["F08"]
["F09"]
["F10"]
["F11"]
["F12"]
["G01"]
["G02"]
["G03"]
["G04"]
["G05"]
["G06"]
["G07"]
["G08"]
["G09"]
["G10"]
["G11"]
["G12"]
["H01"]
["H02"]
["H03"]
["H04"]
["H05"]
["H06"]
["H07"]
["H08"]
["H09"]
["H10"]
["H11"]
["H12"]

{"sheet":"Analysis","index":9,"n_rows":136,"n_cols":21,"rows_kept":136,"cut":false,"hidden":false,"merged":[[30,19,31,21],[72,20,75,20],[76,19,79,19],[80,19,83,19],[84,19,87,19],[88,19,91,19],[132,19,135,19],[76,20,79,20],[80,20,83,20],[84,20,87,20],[88,20,91,20],[92,20,95,20],[92,19,95,19],[96,19,99,19],[120,20,123,20],[124,20,127,20],[128,20,131,20],[132,20,135,20],[96,20,99,20],[100,20,103,20],[104,20,107,20],[108,20,111,20],[112,20,115,20],[116,20,119,20],[100,19,103,19],[72,19,75,19],[116,19,119,19],[120,19,123,19],[116,6,119,6],[120,6,123,6],[124,19,127,19],[128,19,131,19],[104,19,107,19],[108,19,111,19],[112,19,115,19],[88,18,91,18],[92,18,95,18],[96,18,99,18],[116,18,119,18],[120,18,123,18],[124,6,127,6],[128,6,131,6],[124,2,127,2],[128,2,131,2],[132,2,135,2],[104,3,107,3],[108,3,111,3],[112,3,115,3],[116,3,119,3],[120,3,123,3],[124,3,127,3],[128,3,131,3],[104,2,107,2],[108,2,111,2],[112,2,115,2],[116,2,119,2],[120,2,123,2],[132,3,135,3],[132,6,135,6],[104,6,107,6],[108,6,111,6],[112,6,115,6],[84,18,87,18],[92,2,95,2],[96,2,99,2],[100,2,103,2],[72,3,75,3],[76,3,79,3],[80,3,83,3],[84,3,87,3],[88,3,91,3],[92,3,95,3],[96,3,99,3],[72,2,75,2],[76,2,79,2],[80,2,83,2],[84,2,87,2],[88,2,91,2],[100,3,103,3],[92,6,95,6],[96,6,99,6],[100,6,103,6],[0,9,0,10],[26,9,27,9],[72,6,75,6],[76,6,79,6],[80,6,83,6],[84,6,87,6],[88,6,91,6],[132,18,135,18],[14,9,15,9],[16,9,17,9],[18,9,19,9],[20,9,21,9],[22,9,23,9],[24,9,25,9],[41,7,45,7],[124,18,127,18],[128,18,131,18],[100,18,103,18],[104,18,107,18],[108,18,111,18],[112,18,115,18],[72,18,75,18],[76,18,79,18],[80,18,83,18]]}
[null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
[null,null,"Section 1. Review Cq values for DNA Standards"]
[]
[null,null,"- Enter the appropriate information into the fields highlighted in green."]
[null,null,"- Move  \"outliers\" to column G (so these are no longer is used in calculations). Delete the formula in the corresponding row in column I."]
[null,null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between 3.2 and 3.45 is very good  "]
[null,null,"   and 3.1 - 3.6 is acceptable)."]
[null,null,"- If the spacing between any two standards is less than 3.1 cycles and more than 3.6 cycles, those data points (and any library samples falling between those"]
[null,null,"  data points) are not highly reliable."]
[]
[]
[null,null,"Well","Std #","Conc (pM)","Cq","Outliers","Av Cq","Difference","Delta Cq"]
[null,null,"A11",1,20,{"$f":"IF('Cq data input'!$B$14=\"\",\"\",'Cq data input'!$B$14)","v":""},null,{"$f":"AVERAGE(F14:F15)","v":{"$error":"#DIV/0!"}},{"$f":"F14-$H$14","v":{"$error":"#VALUE!"}}]
[null,null,"A12",1,20,{"$f":"IF('Cq data input'!$B$15=\"\",\"\",'Cq data input'!$B$15)","v":""},null,null,{"$f":"F15-$H$14","v":{"$error":"#VALUE!"}},{"$f":"H16-H14","v":{"$error":"#DIV/0!"}}]
[null,null,"B11",2,2,{"$f":"IF('Cq data input'!$B$26=\"\",\"\",'Cq data input'!$B$26)","v":""},null,{"$f":"AVERAGE(F16:F17)","v":{"$error":"#DIV/0!"}},{"$f":"F16-$H$16","v":{"$error":"#VALUE!"}}]
[null,null,"B12",2,2,{"$f":"IF('Cq data input'!$B$27=\"\",\"\",'Cq data input'!$B$27)","v":""},null,null,{"$f":"F17-$H$16","v":{"$error":"#VALUE!"}},{"$f":"H18-H16","v":{"$error":"#DIV/0!"}}]
[null,null,"C11",3,0.2,{"$f":"IF('Cq data input'!$B$38=\"\",\"\",'Cq data input'!$B$38)","v":""},null,{"$f":"AVERAGE(F18:F19)","v":{"$error":"#DIV/0!"}},{"$f":"F18-$H$18","v":{"$error":"#VALUE!"}}]
[null,null,"C12",3,0.2,{"$f":"IF('Cq data input'!$B$39=\"\",\"\",'Cq data input'!$B$39)","v":""},null,null,{"$f":"F19-$H$18","v":{"$error":"#VALUE!"}},{"$f":"H20-H18","v":{"$error":"#DIV/0!"}}]
[null,null,"D11",4,0.02,{"$f":"IF('Cq data input'!$B$50=\"\",\"\",'Cq data input'!$B$50)","v":""},null,{"$f":"AVERAGE(F20:F21)","v":{"$error":"#DIV/0!"}},{"$f":"F20-$H$20","v":{"$error":"#VALUE!"}}]
[null,null,"D12",4,0.02,{"$f":"IF('Cq data input'!$B$51=\"\",\"\",'Cq data input'!$B$51)","v":""},null,null,{"$f":"F21-$H$20","v":{"$error":"#VALUE!"}},{"$f":"H22-H20","v":{"$error":"#DIV/0!"}}]
[null,null,"E11",5,2E-3,{"$f":"IF('Cq data input'!$B$62=\"\",\"\",'Cq data input'!$B$62)","v":""},null,{"$f":"AVERAGE(F22:F23)","v":{"$error":"#DIV/0!"}},{"$f":"F22-$H$22","v":{"$error":"#VALUE!"}}]
[null,null,"E12",5,2E-3,{"$f":"IF('Cq data input'!$B$63=\"\",\"\",'Cq data input'!$B$63)","v":""},null,null,{"$f":"F23-$H$22","v":{"$error":"#VALUE!"}},{"$f":"H24-H22","v":{"$error":"#DIV/0!"}}]
[null,null,"F11",6,2.0000000000000001E-4,{"$f":"IF('Cq data input'!$B$74=\"\",\"\",'Cq data input'!$B$74)","v":""},null,{"$f":"AVERAGE(F24:F25)","v":{"$error":"#DIV/0!"}},{"$f":"F24-$H$24","v":{"$error":"#VALUE!"}}]
[null,null,"F12",6,2.0000000000000001E-4,{"$f":"IF('Cq data input'!$B$75=\"\",\"\",'Cq data input'!$B$75)","v":""},null,null,{"$f":"F25-$H$24","v":{"$error":"#VALUE!"}},{"$f":"H26-H24","v":{"$error":"#DIV/0!"}}]
[null,null,"G11","NTC","-",{"$f":"IF('Cq data input'!$B$86=\"\",\"\",'Cq data input'!$B$86)","v":""},null,{"$f":"AVERAGE(F26:F29)","v":{"$error":"#DIV/0!"}},{"$f":"F26-$H$26","v":{"$error":"#VALUE!"}}]
[null,null,"G12","NTC","-",{"$f":"IF('Cq data input'!$B$87=\"\",\"\",'Cq data input'!$B$87)","v":""},null,null,{"$f":"F27-$H$26","v":{"$error":"#VALUE!"}},{"$f":"H28-H26","v":{"$error":"#DIV/0!"}}]
[null,null,"H11","NTC","-",{"$f":"IF('Cq data input'!$B$98=\"\",\"\",'Cq data input'!$B$98)","v":""},null,null,{"$f":"F28-$H$26","v":{"$error":"#VALUE!"}}]
[null,null,"H12","NTC","-",{"$f":"IF('Cq data input'!$B$99=\"\",\"\",'Cq data input'!$B$99)","v":""},null,null,{"$error":"#VALUE!"}]
[]
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
[null,null,"Section 2. Generate and review the standard curve"]
[]
[]
[null,null,null,null,null,null,null,null,null,null,"Note: if the standard curve equation does not update, click on the line, right click and select"]
[null,null,null,null,null,null,null,null,null,null,"Format Trendline. (Untick and) tick the boxes for \"Display Equation and R-squared value on chart\""]
[]
[]
[null,null,"DNA Standard","Conc in pM","Log conc","Average Cq","Delta Cq"]
[null,null,1,20,{"$f":"LOG(D41)","v":1.3010299956639813},{"$f":"H14","v":{"$error":"#DIV/0!"}},"-"]
[null,null,2,2,0.3010299956639812,{"$f":"H16","v":{"$error":"#DIV/0!"}},{"$f":"F42-F41","v":{"$error":"#DIV/0!"}},"Should be \nbetween \n3.1 and 3.6"]
[null,null,3,0.2,-0.69897000433601875,{"$f":"H18","v":{"$error":"#DIV/0!"}},{"$f":"F43-F42","v":{"$error":"#DIV/0!"}}]
[null,null,4,0.02,-1.6989700043360187,{"$f":"H20","v":{"$error":"#DIV/0!"}},{"$f":"F44-F43","v":{"$error":"#DIV/0!"}}]
[null,null,5,2E-3,-2.6989700043360187,{"$f":"H22","v":{"$error":"#DIV/0!"}},{"$f":"F45-F44","v":{"$error":"#DIV/0!"}}]
[null,null,6,2.0000000000000001E-4,-3.6989700043360187,{"$f":"H24","v":{"$error":"#DIV/0!"}},{"$f":"F46-F45","v":{"$error":"#DIV/0!"}}]
[null,null,"Efficiency:",{"$f":"POWER(10, 1/(-SLOPE(F41:F46, LOG(D41:D46))))-1","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 90 and 110%"]
[null,null,"Slope:",{"$f":"SLOPE(F41:F46, LOG(D41:D46))","v":{"$error":"#DIV/0!"}},"(Calculated)","Value must be the same as on the graph"]
[null,null,"R-squared:",{"$f":"RSQ(F41:F46, -LOG(D41:D46))","v":{"$error":"#DIV/0!"}},"(Calculated)","Should be between 0.99 and 1.00"]
[null,null,"Intercept:",{"$f":"INTERCEPT(F41:F46,E41:E46)","v":{"$error":"#DIV/0!"}}]
[]
[null,null,"If slope =",{"$f":"SLOPE(F41:F46,E41:E46)","v":{"$error":"#DIV/0!"}}]
[null,null,"then efficiency =",{"$f":"POWER(10, (-1/D52))-1","v":{"$error":"#DIV/0!"}},"(Calculated)"]
[]
[]
[null,null,"Note: it is important to type the appropriate values from the straight line equation into the two green blocks,"]
[null,null,"as the table in Section 3 uses these two values to calculate the concentration of the library samples."]
[]
[]
[]
[]
[]
[null,null,"Section 3. Calculate and review library concentrations"]
[]
[null,null,"- Sort the data for your library samples by grouping the Cq values for different dilutions of the same sample together. Enter the appropriate information into the fields highlighted in green (Columns C - G). "]
[null,null,"- Move the outliers  to Column H, so these are no longer is used in calculations. If you move a Cq value (outlier) from column F to H, you have to delete the formula in column J of that row."]
[]
[null,null,"If the average Cq value for a library < than the average Cq value for Std 1, or > than the average Cq value for Std 6, the data from that dilution may not be used in calculations (i.e. you may not extrapolate). If only one dilution of each library was assayed, the library has to"]
[null,null,"requantified using a more appropriate dilution."]
[]
[]
[null,null,"Library #","Sample name","Dilution","Cq","Average fragment length (bp)","Outliers/\noutside curve","Average Cq","Difference","Delta Cq","log\n(concentration)","Average concentration (pM)","Size-adjusted concentration (pM)","Concentration  of undiluted library (pM)","Concentration  of undiluted library (nM)","Conentration of undiluted library (ng/µL)","% Deviation","Working concentration (pM)","Working concentration (nM)","Working concentration (ng/µL)"]
[null,null,1,{"$f":"IF('Chromium Connect Assay Info'!$B$5=\"\",\"\",'Chromium Connect Assay Info'!$B$5)","v":""},{"$f":"'Chromium Connect Assay Info'!$H$1","v":12500},{"$f":"IF('Cq data input'!$B$4=\"\",\"\",'Cq data input'!$B$4)","v":""},{"$f":"IF('Chromium Connect Assay Info'!$C$5=\"\",\"\",'Chromium Connect Assay Info'!$C$5)","v":""},null,{"$f":"AVERAGE(F73:F73)","v":{"$error":"#DIV/0!"}},{"$f":"F73-I73","v":{"$error":"#VALUE!"}},"-",{"$f":"(I73-$D$50)/$D$52","v":{"$error":"#DIV/0!"}},{"$f":"10^L73","v":{"$error":"#DIV/0!"}},{"$f":"M73*(452/G73)","v":{"$error":"#DIV/0!"}},{"$f":"N73*E73","v":{"$error":"#DIV/0!"}},{"$f":"O73/1000","v":{"$error":"#DIV/0!"}},{"$f":"((P73*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O73:O76)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P73:P76)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q73:Q76)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,{"$f":"'Chromium Connect Assay Info'!$H$2","v":62500},{"$f":"IF('Cq data input'!$B$5=\"\",\"\",'Cq data input'!$B$5)","v":""},null,null,{"$error":"#DIV/0!"},{"$error":"#VALUE!"},{"$f":"I74-I73","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M74*(452/G73)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P74*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P74/P73)","v":{"$error":"#DIV/0!"}}]
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[null,null,null,null,{"$f":"'Chromium Connect Assay Info'!$H$4","v":1562500},{"$f":"IF('Cq data input'!$B$83=\"\",\"\",'Cq data input'!$B$83)","v":""},null,null,{"$error":"#DIV/0!"},{"$error":"#VALUE!"},{"$f":"I132-I131","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M132*(452/G129)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P132*10^-12)*(G129*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P132/P129)","v":{"$error":"#DIV/0!"}}]
[null,null,16,{"$f":"IF('Chromium Connect Assay Info'!$B$12=\"\",\"\",'Chromium Connect Assay Info'!$B$12)","v":""},{"$f":"'Chromium Connect Assay Info'!$H$1","v":12500},{"$f":"IF('Cq data input'!$B$92=\"\",\"\",'Cq data input'!$B$92)","v":""},{"$f":"IF('Chromium Connect Assay Info'!$C$12=\"\",\"\",'Chromium Connect Assay Info'!$C$12)","v":""},null,{"$error":"#DIV/0!"},{"$error":"#VALUE!"},"-",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M133*(452/G133)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P133*10^-12)*(G133*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},"-",{"$f":"AVERAGE(O133:O136)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(P133:P136)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(Q133:Q136)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,{"$f":"'Chromium Connect Assay Info'!$H$2","v":62500},{"$f":"IF('Cq data input'!$B$93=\"\",\"\",'Cq data input'!$B$93)","v":""},null,null,{"$error":"#DIV/0!"},{"$error":"#VALUE!"},{"$f":"I134-I133","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M134*(452/G133)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P134*10^-12)*(G133*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P134/P133)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,{"$f":"'Chromium Connect Assay Info'!$H$3","v":312500},{"$f":"IF('Cq data input'!$B$94=\"\",\"\",'Cq data input'!$B$94)","v":""},null,"`",{"$error":"#DIV/0!"},{"$error":"#VALUE!"},{"$f":"I135-I134","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M135*(452/G133)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P135*10^-12)*(G133*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P135/P133)","v":{"$error":"#DIV/0!"}}]
[null,null,null,null,{"$f":"'Chromium Connect Assay Info'!$H$4","v":1562500},{"$f":"IF('Cq data input'!$B$95=\"\",\"\",'Cq data input'!$B$95)","v":""},null,null,{"$error":"#DIV/0!"},{"$error":"#VALUE!"},{"$f":"I136-I135","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"M136*(452/G133)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"((P136*10^-12)*(G133*617.9))*10^-6*10^9*10^3","v":{"$error":"#DIV/0!"}},{"$f":"1-(P136/P133)","v":{"$error":"#DIV/0!"}}]

{"sheet":"Summary","index":10,"n_rows":32,"n_cols":18,"rows_kept":32,"cut":false,"hidden":false,"merged":[[0,17,1,19]]}
[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[null,null,"Library concentrations"]
[]
[null,null,"Sample name","Library Concentration (pM)","Library Concentration (nM)","Library Concentration (ng/µL)"]
[null,null,{"$f":"Analysis!D73","v":""},{"$f":"Analysis!S73","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T73","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U73","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D77","v":""},{"$f":"Analysis!S77","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T77","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U77","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D81","v":""},{"$f":"Analysis!S81","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T81","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U81","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D85","v":""},{"$f":"Analysis!S85","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T85","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U85","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D89","v":""},{"$f":"Analysis!S89","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T89","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U89","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D93","v":""},{"$f":"Analysis!S93","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T93","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U93","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D97","v":""},{"$f":"Analysis!S97","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T97","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U97","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D101","v":""},{"$f":"Analysis!S101","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T101","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U101","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D105","v":""},{"$f":"Analysis!S105","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T105","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U105","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D109","v":""},{"$f":"Analysis!S109","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T109","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U109","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D113","v":""},{"$f":"Analysis!S113","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T113","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U113","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D117","v":""},{"$f":"Analysis!S117","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T117","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U117","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D121","v":""},{"$f":"Analysis!S121","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T121","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U121","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D125","v":""},{"$f":"Analysis!S125","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T125","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U125","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D129","v":""},{"$f":"Analysis!S129","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T129","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U129","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"Analysis!D133","v":""},{"$f":"Analysis!S133","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!T133","v":{"$error":"#DIV/0!"}},{"$f":"Analysis!U133","v":{"$error":"#DIV/0!"}}]
[]
[null,null,"Library ave concentrations and yields"]
[]
[null,null,"Sample name","Ave Library Concentration (pM)","Ave Library Concentration (nM)","Stnd Dev of Library Conc (nM)","Ave Conc of library (ng/µL)","Library volume (µL)","Available amount of library (ng)"]
[null,null,{"$f":"C5","v":""},{"$f":"AVERAGE(D5,D13)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$f":"STDEV(E5,E13)","v":{"$error":"#DIV/0!"}},{"$f":"AVERAGE(F5,F13)","v":{"$error":"#DIV/0!"}},{"$f":"25","v":25},{"$f":"G25*H25","v":{"$error":"#DIV/0!"}}]
[null,null,{"$f":"C6","v":""},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"STDEV(E6,E14)","v":{"$error":"#DIV/0!"}},{"$error":"#DIV/0!"},{"$f":"25","v":25},{"$f":"G26*H26","v":{"$error":"#DIV/0!"}}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$f":"25","v":25},{"$error":"#DIV/0!"}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},25,{"$error":"#DIV/0!"}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},25,{"$error":"#DIV/0!"}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},25,{"$error":"#DIV/0!"}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},25,{"$error":"#DIV/0!"}]
[null,null,"",{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},{"$error":"#DIV/0!"},25,{"$error":"#DIV/0!"}]

{"sheet":"Example Assay Info","index":11,"n_rows":12,"n_cols":10,"rows_kept":12,"cut":false,"hidden":false,"merged":[[0,9,1,11]]}
["Fill in the sample names and corresponding average library sizes for this experiment",null,null,null,null,null,"Dilution 1",12500,null,"CG000157 Rev A"]
[null,null,null,null,null,null,"Dilution 2",62500]
[null,null,null,null,null,null,"Dilution 3",312500]
[null,"Sample name","ave lib size (bp)(BioA)",null,null,null,"Dilution 4",1562500]
["Sample 1","Connect_1",466]
["Sample 2","Connect_2",458]
["Sample 3","Connect_3",458]
["Sample 4","Connect_4",465]
["Sample 5","Connect_5",467]
["Sample 6","Connect_6",470]
["Sample 7","Connect_7",465]
["Sample 8","Connect_8",470]

{"sheet":"Example Cq data","index":12,"n_rows":99,"n_cols":10,"rows_kept":99,"cut":false,"hidden":false,"merged":[[0,9,1,11]]}
["Copy and paste the Cq values obtained for this experiment into cells B4-B99",null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
["Well","Cq"]
["A01",14.4]
["A02",16.84]
["A03",19.309999999999999]
["A04",21.86]
["A05",14.35]
["A06",16.89]
["A07",19.52]
["A08",21.94]
["A09"]
["A10"]
["A11",9.8000000000000007]
["A12",9.73]
["B01",14.9]
["B02",17.36]
["B03",19.89]
["B04",22.28]
["B05",14.93]
["B06",17.399999999999999]
["B07",20]
["B08",22.54]
["B09"]
["B10"]
["B11",13.47]
["B12",13.6]
["C01",14.47]
["C02",16.96]
["C03",19.39]
["C04",21.98]
["C05",14.5]
["C06",17.059999999999999]
["C07",19.54]
["C08",22.04]
["C09"]
["C10"]
["C11",17.04]
["C12",17.04]
["D01",14.68]
["D02",17.149999999999999]
["D03",19.59]
["D04",22]
["D05",14.6]
["D06",17.170000000000002]
["D07",19.64]
["D08",22.17]
["D09"]
["D10"]
["D11",20.52]
["D12",20.64]
["E01",14.26]
["E02",16.79]
["E03",19.170000000000002]
["E04",21.7]
["E05",14.4]
["E06",16.77]
["E07",19.27]
["E08",21.8]
["E09"]
["E10"]
["E11",23.83]
["E12",23.83]
["F01",14.78]
["F02",17.11]
["F03",19.68]
["F04",22.17]
["F05",14.74]
["F06",17.22]
["F07",19.72]
["F08",22.24]
["F09"]
["F10"]
["F11",27.52]
["F12",27.4]
["G01",14.53]
["G02",17.04]
["G03",19.48]
["G04",22.06]
["G05",14.52]
["G06",17.12]
["G07",19.59]
["G08",22.13]
["G09"]
["G10"]
["G11"]
["G12"]
["H01",14.56]
["H02",17.07]
["H03",19.45]
["H04",21.95]
["H05",14.51]
["H06",17.079999999999998]
["H07",19.53]
["H08",22.08]
["H09"]
["H10"]
["H11"]
["H12"]

{"sheet":"Example Analysis","index":13,"n_rows":136,"n_cols":21,"rows_kept":136,"cut":false,"hidden":false,"merged":[[9,19,10,21],[14,9,15,9],[16,9,17,9],[18,9,19,9],[20,9,21,9],[22,9,23,9],[24,9,25,9],[72,19,75,19],[72,20,75,20],[76,2,79,2],[76,3,79,3],[76,6,79,6],[76,18,79,18],[76,19,79,19],[76,20,79,20],[26,9,27,9],[41,7,45,7],[72,2,75,2],[72,3,75,3],[72,6,75,6],[72,18,75,18],[84,2,87,2],[84,3,87,3],[84,6,87,6],[84,18,87,18],[84,19,87,19],[84,20,87,20],[80,2,83,2],[80,3,83,3],[80,6,83,6],[80,18,83,18],[80,19,83,19],[80,20,83,20],[92,2,95,2],[92,3,95,3],[92,6,95,6],[92,18,95,18],[92,19,95,19],[92,20,95,20],[88,2,91,2],[88,3,91,3],[88,6,91,6],[88,18,91,18],[88,19,91,19],[88,20,91,20],[100,2,103,2],[100,3,103,3],[100,6,103,6],[100,18,103,18],[100,19,103,19],[100,20,103,20],[96,2,99,2],[96,3,99,3],[96,6,99,6],[96,18,99,18],[96,19,99,19],[96,20,99,20],[108,2,111,2],[108,3,111,3],[108,6,111,6],[108,18,111,18],[108,19,111,19],[108,20,111,20],[104,2,107,2],[104,3,107,3],[104,6,107,6],[104,18,107,18],[104,19,107,19],[104,20,107,20],[120,20,123,20],[116,2,119,2],[116,3,119,3],[116,6,119,6],[116,18,119,18],[116,19,119,19],[116,20,119,20],[112,2,115,2],[112,3,115,3],[112,6,115,6],[112,18,115,18],[112,19,115,19],[112,20,115,20],[0,9,0,10],[132,2,135,2],[132,3,135,3],[132,6,135,6],[132,18,135,18],[132,19,135,19],[132,20,135,20],[128,2,131,2],[128,3,131,3],[128,6,131,6],[128,18,131,18],[128,19,131,19],[128,20,131,20],[124,2,127,2],[124,3,127,3],[124,6,127,6],[124,18,127,18],[124,19,127,19],[124,20,127,20],[120,2,123,2],[120,3,123,3],[120,6,123,6],[120,18,123,18],[120,19,123,19]]}
[null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
[null,null,"Section 1. Review Cq values for DNA Standards"]
[]
[null,null,"- Enter the appropriate information into the fields highlighted in green."]
[null,null,"- Move  \"outliers\" to column G (so these are no longer is used in calculations). Delete the formula in the corresponding row in column I."]
[null,null,"- The average Cq value for each DNA Standard should be ~3.3 cycles later than the DNA Standard that is 10-fold more concentrated (between 3.2 and 3.45 is very good  "]
[null,null,"   and 3.1 - 3.6 is acceptable)."]
[null,null,"- If the spacing between any two standards is less than 3.1 cycles and more than 3.6 cycles, those data points (and any library samples falling between those"]
[null,null,"  data points) are not highly reliable.",null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[]
[]
[null,null,"Well","Std #","Conc (pM)","Cq","Outliers","Av Cq","Difference","Delta Cq"]
[null,null,"A11",1,20,{"$f":"IF('Example Cq data'!$B$14=\"\",\"\",'Example Cq data'!$B$14)","v":9.8000000000000007},null,{"$f":"AVERAGE(F14:F15)","v":9.7650000000000006},{"$f":"F14-$H$14","v":3.5000000000000142E-2}]
[null,null,"A12",1,20,{"$f":"IF('Example Cq data'!$B$15=\"\",\"\",'Example Cq data'!$B$15)","v":9.73},null,null,{"$f":"F15-$H$14","v":-3.5000000000000142E-2},{"$f":"H16-H14","v":3.7699999999999996}]
[null,null,"B11",2,2,{"$f":"IF('Example Cq data'!$B$26=\"\",\"\",'Example Cq data'!$B$26)","v":13.47},null,{"$f":"AVERAGE(F16:F17)","v":13.535},{"$f":"F16-$H$16","v":-6.4999999999999503E-2}]
[null,null,"B12",2,2,{"$f":"IF('Example Cq data'!$B$27=\"\",\"\",'Example Cq data'!$B$27)","v":13.6},null,null,{"$f":"F17-$H$16","v":6.4999999999999503E-2},{"$f":"H18-H16","v":3.504999999999999}]
[null,null,"C11",3,0.2,{"$f":"IF('Example Cq data'!$B$38=\"\",\"\",'Example Cq data'!$B$38)","v":17.04},null,{"$f":"AVERAGE(F18:F19)","v":17.04},{"$f":"F18-$H$18","v":0}]
[null,null,"C12",3,0.2,{"$f":"IF('Example Cq data'!$B$39=\"\",\"\",'Example Cq data'!$B$39)","v":17.04},null,null,{"$f":"F19-$H$18","v":0},{"$f":"H20-H18","v":3.5399999999999991}]
[null,null,"D11",4,0.02,{"$f":"IF('Example Cq data'!$B$50=\"\",\"\",'Example Cq data'!$B$50)","v":20.52},null,{"$f":"AVERAGE(F20:F21)","v":20.58},{"$f":"F20-$H$20","v":-5.9999999999998721E-2}]
[null,null,"D12",4,0.02,{"$f":"IF('Example Cq data'!$B$51=\"\",\"\",'Example Cq data'!$B$51)","v":20.64},null,null,{"$f":"F21-$H$20","v":6.0000000000002274E-2},{"$f":"H22-H20","v":3.25}]
[null,null,"E11",5,2E-3,{"$f":"IF('Example Cq data'!$B$62=\"\",\"\",'Example Cq data'!$B$62)","v":23.83},null,{"$f":"AVERAGE(F22:F23)","v":23.83},{"$f":"F22-$H$22","v":0}]
[null,null,"E12",5,2E-3,{"$f":"IF('Example Cq data'!$B$63=\"\",\"\",'Example Cq data'!$B$63)","v":23.83},null,null,{"$f":"F23-$H$22","v":0},{"$f":"H24-H22","v":3.6300000000000026}]
[null,null,"F11",6,2.0000000000000001E-4,{"$f":"IF('Example Cq data'!$B$74=\"\",\"\",'Example Cq data'!$B$74)","v":27.52},null,{"$f":"AVERAGE(F24:F25)","v":27.46},{"$f":"F24-$H$24","v":5.9999999999998721E-2}]
[null,null,"F12",6,2.0000000000000001E-4,{"$f":"IF('Example Cq data'!$B$75=\"\",\"\",'Example Cq data'!$B$75)","v":27.4},null,null,{"$f":"F25-$H$24","v":-6.0000000000002274E-2},{"$f":"H26-H24","v":{"$error":"#DIV/0!"}}]
[null,null,"G11","NTC","-",{"$f":"IF('Example Cq data'!$B$86=\"\",\"\",'Example Cq data'!$B$86)","v":""},null,{"$f":"AVERAGE(F26:F29)","v":{"$error":"#DIV/0!"}},{"$f":"F26-$H$26","v":{"$error":"#VALUE!"}}]
[null,null,"G12","NTC","-",{"$f":"IF('Example Cq data'!$B$87=\"\",\"\",'Example Cq data'!$B$87)","v":""},null,null,{"$f":"F27-$H$26","v":{"$error":"#VALUE!"}},{"$f":"H28-H26","v":{"$error":"#DIV/0!"}}]
[null,null,"H11","NTC","-",{"$f":"IF('Example Cq data'!$B$98=\"\",\"\",'Example Cq data'!$B$98)","v":""},null,null,{"$f":"F28-$H$26","v":{"$error":"#VALUE!"}}]
[null,null,"H12","NTC","-",{"$f":"IF('Example Cq data'!$B$99=\"\",\"\",'Example Cq data'!$B$99)","v":""},null,null,{"$error":"#VALUE!"}]
[]
[]
[]
[null,null,"Section 2. Generate and review the standard curve"]
[]
[]
[null,null,null,null,null,null,null,null,null,null,"Note: if the standard curve equation does not update, click on the line, right click and select"]
[null,null,null,null,null,null,null,null,null,null,"Format Trendline. (Untick and) tick the boxes for \"Display Equation and R-squared value on chart\""]
[]
[]
[null,null,"DNA Standard","Conc in pM","Log conc","Average Cq","Delta Cq"]
[null,null,1,20,{"$f":"LOG(D41)","v":1.3010299956639813},{"$f":"H14","v":9.7650000000000006},"-"]
[null,null,2,2,0.3010299956639812,{"$f":"H16","v":13.535},{"$f":"F42-F41","v":3.7699999999999996},"Should be \nbetween \n3.1 and 3.6"]
[null,null,3,0.2,-0.69897000433601875,{"$f":"H18","v":17.04},{"$f":"F43-F42","v":3.504999999999999}]
[null,null,4,0.02,-1.6989700043360187,{"$f":"H20","v":20.58},{"$f":"F44-F43","v":3.5399999999999991}]
[null,null,5,2E-3,-2.6989700043360187,{"$f":"H22","v":23.83},{"$f":"F45-F44","v":3.25}]
[null,null,6,2.0000000000000001E-4,-3.6989700043360187,{"$f":"H24","v":27.46},{"$f":"F46-F45","v":3.6300000000000026}]
[null,null,"Efficiency:",{"$f":"POWER(10, 1/(-SLOPE(F41:F46, LOG(D41:D46))))-1","v":0.92656815569611917},"(Calculated)","Should be between 90 and 110%"]
[null,null,"Slope:",{"$f":"SLOPE(F41:F46, LOG(D41:D46))","v":-3.5114285714285707},"(Calculated)","Value must be the same as on the graph"]
[null,null,"R-squared:",{"$f":"RSQ(F41:F46, -LOG(D41:D46))","v":0.99963564763852863},"(Calculated)","Should be between 0.99 and 1.00"]
[null,null,"Intercept:",{"$f":"INTERCEPT(F41:F46,E41:E46)","v":14.491569137155334}]
[]
[null,null,"If slope =",{"$f":"SLOPE(F41:F46,E41:E46)","v":-3.5114285714285707}]
[null,null,"then efficiency =",{"$f":"POWER(10, (-1/D52))-1","v":0.92656815569611917},"(Calculated)"]
[]
[]
[null,null,"Note: it is important to type the appropriate values from the straight line equation into the two green blocks,"]
[null,null,"as the table in Section 3 uses these two values to calculate the concentration of the library samples."]
[]
[]
[]
[]
[]
[null,null,"Section 3. Calculate and review library concentrations"]
[]
[null,null,"- Sort the data for your library samples by grouping the Cq values for different dilutions of the same sample together. Enter the appropriate information into the fields highlighted in green (Columns C - G). "]
[null,null,"- Move the outliers  to Column H, so these are no longer is used in calculations. If you move a Cq value (outlier) from column F to H, you have to delete the formula in column J of that row."]
[]
[null,null,"If the average Cq value for a library < than the average Cq value for Std 1, or > than the average Cq value for Std 6, the data from that dilution may not be used in calculations (i.e. you may not extrapolate). If only one dilution of each library was assayed, the library has to"]
[null,null,"requantified using a more appropriate dilution."]
[]
[]
[null,null,"Library #","Sample name","Dilution","Cq","Average fragment length (bp)","Outliers/\noutside curve","Average Cq","Difference","Delta Cq","log\n(concentration)","Average concentration (pM)","Size-adjusted concentration (pM)","Concentration  of undiluted library (pM)","Concentration  of undiluted library (nM)","Conentration of undiluted library (ng/µL)","% Deviation","Working concentration (pM)","Working concentration (nM)","Working concentration (ng/µL)"]
[null,null,1,{"$f":"IF('Example Assay Info'!$B$5=\"\",\"\",'Example Assay Info'!$B$5)","v":"Connect_1"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$4=\"\",\"\",'Example Cq data'!$B$4)","v":14.4},{"$f":"IF('Example Assay Info'!$C$5=\"\",\"\",'Example Assay Info'!$C$5)","v":466},null,{"$f":"AVERAGE(F73:F73)","v":14.4},{"$f":"F73-I73","v":0},"-",{"$f":"(I73-$D$50)/$D$52","v":2.6077459726905537E-2},{"$f":"10^L73","v":1.0618849355715225},{"$f":"M73*(452/G73)","v":1.0299828130436226},{"$f":"N73*E73","v":12874.785163045282},{"$f":"O73/1000","v":12.874785163045283},{"$f":"((P73*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":3.707183664546486},"-",{"$f":"AVERAGE(O73:O76)","v":12704.62486618058},{"$f":"AVERAGE(P73:P76)","v":12.704624866180581},{"$f":"AVERAGE(Q73:Q76)","v":3.6581874704428481}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$5=\"\",\"\",'Example Cq data'!$B$5)","v":16.84},null,null,16.84,0,{"$f":"I74-I73","v":2.4399999999999995},-0.66879642147732554,0.21438953312830294,{"$f":"M74*(452/G73)","v":0.20794864586693762},12996.790366683601,12.996790366683602,{"$f":"((P74*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":3.7423140136893887},{"$f":"1-(P74/P73)","v":-9.4762904462679654E-3}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$6=\"\",\"\",'Example Cq data'!$B$6)","v":19.309999999999999},null,"`",19.309999999999999,0,{"$f":"I75-I74","v":2.4699999999999989},-1.372213834007838,4.2441054504913693E-2,{"$f":"M75*(452/G73)","v":4.1166001365281091E-2},12864.37542665034,12.864375426650341,{"$f":"((P75*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":3.7041862704752959},{"$f":"1-(P75/P73)","v":8.0853670668012967E-4}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$7=\"\",\"\",'Example Cq data'!$B$7)","v":21.86},null,null,21.86,0,{"$f":"I76-I75","v":2.5500000000000007},-2.0984139967417685,7.9723435113456739E-3,{"$f":"M76*(452/G73)","v":7.7328310453395801E-3},12082.548508343094,12.082548508343095,{"$f":"((P76*10^-12)*(G73*617.9))*10^-6*10^9*10^3","v":3.4790659330602218},{"$f":"1-(P76/P73)","v":6.1533970832861606E-2}]
[null,null,2,{"$f":"IF('Example Assay Info'!$B$6=\"\",\"\",'Example Assay Info'!$B$6)","v":"Connect_2"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$16=\"\",\"\",'Example Cq data'!$B$16)","v":14.9},{"$f":"IF('Example Assay Info'!$C$6=\"\",\"\",'Example Assay Info'!$C$6)","v":458},null,14.9,0,"-",-0.11631472904445332,0.76504198738329054,{"$f":"M77*(452/G77)","v":0.75501960326909889},9437.745040863736,9.4377450408637351,{"$f":"((P77*10^-12)*(G77*617.9))*10^-6*10^9*10^3","v":2.670864858623363},"-",{"$f":"AVERAGE(O77:O80)","v":9280.7501869201496},{"$f":"AVERAGE(P77:P80)","v":9.2807501869201499},{"$f":"AVERAGE(Q77:Q80)","v":2.626435597548066}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$17=\"\",\"\",'Example Cq data'!$B$17)","v":17.36},null,null,17.36,0,{"$f":"I78-I77","v":2.4599999999999991},-0.81688429779953864,0.1524458837125324,{"$f":"M78*(452/G77)","v":0.15044877606564333},9403.0485041027077,9.4030485041027081,{"$f":"((P78*10^-12)*(G77*617.9))*10^-6*10^9*10^3","v":2.661045801173759},{"$f":"1-(P78/P77)","v":3.676358771168009E-3}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$18=\"\",\"\",'Example Cq data'!$B$18)","v":19.89},null,"`",19.89,0,{"$f":"I79-I78","v":2.5300000000000011},-1.5373887729826148,2.9014241863431184E-2,{"$f":"M79*(452/G77)","v":2.8634142625045621E-2},8948.169570326756,8.9481695703267565,{"$f":"((P79*10^-12)*(G77*617.9))*10^-6*10^9*10^3","v":2.532315881697246},{"$f":"1-(P79/P77)","v":5.1874199654388331E-2}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$19=\"\",\"\",'Example Cq data'!$B$19)","v":22.28},null,null,22.28,0,{"$f":"I80-I79","v":2.3900000000000006},-2.2180234353097101,6.053082103551757E-3,{"$f":"M80*(452/G77)","v":5.9737840847279349E-3},9334.0376323873988,9.3340376323873997,{"$f":"((P80*10^-12)*(G77*617.9))*10^-6*10^9*10^3","v":2.641515848697896},{"$f":"1-(P80/P77)","v":1.0988579160308043E-2}]
[null,null,3,{"$f":"IF('Example Assay Info'!$B$7=\"\",\"\",'Example Assay Info'!$B$7)","v":"Connect_3"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$28=\"\",\"\",'Example Cq data'!$B$28)","v":14.47},{"$f":"IF('Example Assay Info'!$C$7=\"\",\"\",'Example Assay Info'!$C$7)","v":458},null,14.47,0,"-",6.1425532989152149E-3,1.0142442477527986,{"$f":"M81*(452/G81)","v":1.0009572052058187},12511.965065072733,12.511965065072733,{"$f":"((P81*10^-12)*(G81*617.9))*10^-6*10^9*10^3","v":3.5408635918784661},"-",{"$f":"AVERAGE(O81:O84)","v":12129.63558869931},{"$f":"AVERAGE(P81:P84)","v":12.12963558869931},{"$f":"AVERAGE(Q81:Q84)","v":3.4326650382578454}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$29=\"\",\"\",'Example Cq data'!$B$29)","v":16.96},null,null,16.96,0,{"$f":"I82-I81","v":2.4900000000000002},-0.70297054678245197,0.19816614146312739,{"$f":"M82*(452/G81)","v":0.19557007847452745},12223.129904657966,12.223129904657966,{"$f":"((P82*10^-12)*(G81*617.9))*10^-6*10^9*10^3","v":3.4591237613843768},{"$f":"1-(P82/P81)","v":2.3084716022829466E-2}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$30=\"\",\"\",'Example Cq data'!$B$30)","v":19.39},null,"`",19.39,0,{"$f":"I83-I82","v":2.4299999999999997},-1.3949965842112559,4.0272020176547914E-2,{"$f":"M83*(452/G81)","v":3.9744439126200126E-2},12420.137226937539,12.420137226937539,{"$f":"((P83*10^-12)*(G81*617.9))*10^-6*10^9*10^3","v":3.5148764789763152},{"$f":"1-(P83/P81)","v":7.3392019285229981E-3}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$31=\"\",\"\",'Example Cq data'!$B$31)","v":21.98},null,null,21.98,0,{"$f":"I84-I83","v":2.59},-2.132588122046895,7.3690563574132159E-3,{"$f":"M84*(452/G81)","v":7.2725185012025625E-3},11363.310158129005,11.363310158129005,{"$f":"((P84*10^-12)*(G81*617.9))*10^-6*10^9*10^3","v":3.215796320792224},{"$f":"1-(P84/P81)","v":9.1804516794105284E-2}]
[null,null,4,{"$f":"IF('Example Assay Info'!$B$8=\"\",\"\",'Example Assay Info'!$B$8)","v":"Connect_4"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$40=\"\",\"\",'Example Cq data'!$B$40)","v":14.68},{"$f":"IF('Example Assay Info'!$C$8=\"\",\"\",'Example Assay Info'!$C$8)","v":465},null,14.68,0,"-",-5.3662165985055243E-2,0.88376710807770464,{"$f":"M85*(452/G85)","v":0.85905964054004835},10738.245506750605,10.738245506750605,{"$f":"((P85*10^-12)*(G85*617.9))*10^-6*10^9*10^3","v":3.0853502828588573},"-",{"$f":"AVERAGE(O85:O88)","v":10785.768433162433},{"$f":"AVERAGE(P85:P88)","v":10.785768433162433},{"$f":"AVERAGE(Q85:Q88)","v":3.099004736405746}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$41=\"\",\"\",'Example Cq data'!$B$41)","v":17.149999999999999},null,null,17.149999999999999,0,{"$f":"I86-I85","v":2.4699999999999989},-0.75707957851556773,0.17495260825596778,{"$f":"M86*(452/G85)","v":0.17006146006816653},10628.841254260407,10.628841254260408,{"$f":"((P86*10^-12)*(G85*617.9))*10^-6*10^9*10^3","v":3.0539158701184901},{"$f":"1-(P86/P85)","v":1.0188280052027099E-2}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$42=\"\",\"\",'Example Cq data'!$B$42)","v":19.59},null,"`",19.59,0,{"$f":"I87-I86","v":2.4400000000000013},-1.4519534597197992,3.5322101997226671E-2,{"$f":"M87*(452/G85)","v":3.4334602371497755E-2},10729.563241093048,10.729563241093048,{"$f":"((P87*10^-12)*(G85*617.9))*10^-6*10^9*10^3","v":3.082855663902198},{"$f":"1-(P87/P85)","v":8.0853670668068478E-4}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$43=\"\",\"\",'Example Cq data'!$B$43)","v":22},null,null,22,0,{"$f":"I88-I87","v":2.41},-2.1382838095977492,7.2730435889610439E-3,{"$f":"M88*(452/G85)","v":7.0697111875492298E-3},11046.423730545672,11.046423730545671,{"$f":"((P88*10^-12)*(G85*617.9))*10^-6*10^9*10^3","v":3.1738971287434388},{"$f":"1-(P88/P85)","v":-2.8699122552313439E-2}]
[null,null,5,{"$f":"IF('Example Assay Info'!$B$9=\"\",\"\",'Example Assay Info'!$B$9)","v":"Connect_5"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$52=\"\",\"\",'Example Cq data'!$B$52)","v":14.26},{"$f":"IF('Example Assay Info'!$C$9=\"\",\"\",'Example Assay Info'!$C$9)","v":467},null,14.26,0,"-",6.5947272582886185E-2,1.1639847021882994,{"$f":"M89*(452/G89)","v":1.126597613252915},14082.470165661438,14.082470165661437,{"$f":"((P89*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":4.0636277332741484},"-",{"$f":"AVERAGE(O89:O92)","v":13736.290186047974},{"$f":"AVERAGE(P89:P92)","v":13.736290186047972},{"$f":"AVERAGE(Q89:Q92)","v":3.9637342806828726}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$53=\"\",\"\",'Example Cq data'!$B$53)","v":16.79},null,null,16.79,0,{"$f":"I90-I89","v":2.5299999999999994},-0.65455720260018946,0.2215352284507037,{"$f":"M90*(452/G89)","v":0.21441953588804727},13401.220993002955,13.401220993002955,{"$f":"((P90*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":3.8670469488862369},{"$f":"1-(P90/P89)","v":4.8375687265408418E-2}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$54=\"\",\"\",'Example Cq data'!$B$54)","v":19.170000000000002},null,"`",19.170000000000002,0,{"$f":"I91-I90","v":2.3800000000000026},-1.3323440211518585,4.6521743113222526E-2,{"$f":"M91*(452/G89)","v":4.502746870915756E-2},14071.083971611737,14.071083971611737,{"$f":"((P91*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":4.0603421410895031},{"$f":"1-(P91/P89)","v":8.0853670668268318E-4}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$55=\"\",\"\",'Example Cq data'!$B$55)","v":21.7},null,null,21.7,0,{"$f":"I92-I91","v":2.5299999999999976},-2.0528484963349336,8.8542443634671321E-3,{"$f":"M92*(452/G89)","v":8.5698467929060888E-3},13390.385613915763,13.390385613915763,{"$f":"((P92*10^-12)*(G89*617.9))*10^-6*10^9*10^3","v":3.863920299481602},{"$f":"1-(P92/P89)","v":4.9145110453224783E-2}]
[null,null,6,{"$f":"IF('Example Assay Info'!$B$10=\"\",\"\",'Example Assay Info'!$B$10)","v":"Connect_6"},{"$f":"'Example Assay Info'!$H$1","v":12500},{"$f":"IF('Example Cq data'!$B$64=\"\",\"\",'Example Cq data'!$B$64)","v":14.78},{"$f":"IF('Example Assay Info'!$C$10=\"\",\"\",'Example Assay Info'!$C$10)","v":470},null,14.78,0,"-",-8.2140603739326912E-2,0.82767415910538478,{"$f":"M93*(452/G93)","v":0.7959759998204976},9949.6999977562209,9.9496999977562215,{"$f":"((P93*10^-12)*(G93*617.9))*10^-6*10^9*10^3","v":2.8895222254483772},"-",{"$f":"AVERAGE(O93:O96)","v":10132.013485045081},{"$f":"AVERAGE(P93:P96)","v":10.132013485045082},{"$f":"AVERAGE(Q93:Q96)","v":2.9424684322323968}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$2","v":62500},{"$f":"IF('Example Cq data'!$B$65=\"\",\"\",'Example Cq data'!$B$65)","v":17.11},null,null,17.11,0,{"$f":"I94-I93","v":2.33},-0.74568820341385922,0.17960225972557037,{"$f":"M94*(452/G93)","v":0.1727238753105485},10795.242206909281,10.795242206909281,{"$f":"((P94*10^-12)*(G93*617.9))*10^-6*10^9*10^3","v":3.1350786750351447},{"$f":"1-(P94/P93)","v":-8.4981678778630387E-2}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$3","v":312500},{"$f":"IF('Example Cq data'!$B$66=\"\",\"\",'Example Cq data'!$B$66)","v":19.68},null,"`",19.68,0,{"$f":"I95-I94","v":2.5700000000000003},-1.4775840536986438,3.3297831118541871E-2,{"$f":"M95*(452/G93)","v":3.2022595033150907E-2},10007.060947859658,10.007060947859658,{"$f":"((P95*10^-12)*(G93*617.9))*10^-6*10^9*10^3","v":2.9061805910507665},{"$f":"1-(P95/P93)","v":-5.765093431598256E-3}]
[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$67=\"\",\"\",'Example Cq data'!$B$67)","v":22.17},null,null,22.17,0,{"$f":"I96-I95","v":2.490000000000002},-2.1866971537800115,6.5058320285988347E-3,{"$f":"M96*(452/G93)","v":6.2566725040993044E-3},9776.0507876551637,9.7760507876551639,{"$f":"((P96*10^-12)*(G93*617.9))*10^-6*10^9*10^3","v":2.8390922373952989},{"$f":"1-(P96/P93)","v":1.7452708135945572E-2}]
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[null,null,null,null,{"$f":"'Example Assay Info'!$H$4","v":1562500},{"$f":"IF('Example Cq data'!$B$71=\"\",\"\",'Example Cq data'!$B$71)","v":22.24},null,null,22.24,0,{"$f":"I128-I127","v":2.5199999999999996},-2.2066320602080007,6.213952652319046E-3,{"$f":"M128*(452/G125)","v":5.9759714869110818E-3},9337.4554482985659,9.3374554482985666,{"$f":"((P128*10^-12)*(G125*617.9))*10^-6*10^9*10^3","v":2.7117184491067321},{"$f":"1-(P128/P125)","v":8.5829544609920316E-2}]
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[null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,null,"CG000157 Rev A"]
[null,null,"Library concentrations"]
[]
[null,null,"Sample name","Library Concentration (pM)","Library Concentration (nM)","Library Concentration (ng/µL)"]
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[null,null,{"$f":"'Example Analysis'!D81","v":"Connect_3"},{"$f":"'Example Analysis'!S81","v":12129.63558869931},{"$f":"'Example Analysis'!T81","v":12.12963558869931},{"$f":"'Example Analysis'!U81","v":3.4326650382578454}]
[null,null,{"$f":"'Example Analysis'!D85","v":"Connect_4"},{"$f":"'Example Analysis'!S85","v":10785.768433162433},{"$f":"'Example Analysis'!T85","v":10.785768433162433},{"$f":"'Example Analysis'!U85","v":3.099004736405746}]
[null,null,{"$f":"'Example Analysis'!D89","v":"Connect_5"},{"$f":"'Example Analysis'!S89","v":13736.290186047974},{"$f":"'Example Analysis'!T89","v":13.736290186047972},{"$f":"'Example Analysis'!U89","v":3.9637342806828726}]
[null,null,{"$f":"'Example Analysis'!D93","v":"Connect_6"},{"$f":"'Example Analysis'!S93","v":10132.013485045081},{"$f":"'Example Analysis'!T93","v":10.132013485045082},{"$f":"'Example Analysis'!U93","v":2.9424684322323968}]
[null,null,{"$f":"'Example Analysis'!D97","v":"Connect_7"},{"$f":"'Example Analysis'!S97","v":11356.087476180925},{"$f":"'Example Analysis'!T97","v":11.356087476180926},{"$f":"'Example Analysis'!U97","v":3.2628707999624695}]
[null,null,{"$f":"'Example Analysis'!D101","v":"Connect_8"},{"$f":"'Example Analysis'!S101","v":11376.293811018651},{"$f":"'Example Analysis'!T101","v":11.376293811018652},{"$f":"'Example Analysis'!U101","v":3.3038236145393594}]
[null,null,{"$f":"'Example Analysis'!D105","v":"Connect_1"},{"$f":"'Example Analysis'!S105","v":12138.992344775636},{"$f":"'Example Analysis'!T105","v":12.138992344775636},{"$f":"'Example Analysis'!U105","v":3.4953184503439783}]
[null,null,{"$f":"'Example Analysis'!D109","v":"Connect_2"},{"$f":"'Example Analysis'!S109","v":8652.4726824678201},{"$f":"'Example Analysis'!T109","v":8.6524726824678204},{"$f":"'Example Analysis'!U109","v":2.4486341946875649}]
[null,null,{"$f":"'Example Analysis'!D113","v":"Connect_3"},{"$f":"'Example Analysis'!S113","v":11474.275983396739},{"$f":"'Example Analysis'!T113","v":11.47427598339674},{"$f":"'Example Analysis'!U113","v":3.2471994496045067}]
[null,null,{"$f":"'Example Analysis'!D117","v":"Connect_4"},{"$f":"'Example Analysis'!S117","v":10517.90155170258},{"$f":"'Example Analysis'!T117","v":10.517901551702579},{"$f":"'Example Analysis'!U117","v":3.0220402864906157}]
[null,null,{"$f":"'Example Analysis'!D121","v":"Connect_5"},{"$f":"'Example Analysis'!S121","v":13035.95782420805},{"$f":"'Example Analysis'!T121","v":13.035957824208047},{"$f":"'Example Analysis'!U121","v":3.761646864582997}]
[null,null,{"$f":"'Example Analysis'!D125","v":"Connect_6"},{"$f":"'Example Analysis'!S125","v":9835.8908387487645},{"$f":"'Example Analysis'!T125","v":9.8358908387487638},{"$f":"'Example Analysis'!U125","v":2.8564705661535452}]
[null,null,{"$f":"'Example Analysis'!D129","v":"Connect_7"},{"$f":"'Example Analysis'!S129","v":10909.863959023001},{"$f":"'Example Analysis'!T129","v":10.909863959022999},{"$f":"'Example Analysis'!U129","v":3.1346602972303446}]
[null,null,{"$f":"'Example Analysis'!D133","v":"Connect_8"},{"$f":"'Example Analysis'!S133","v":11074.585287676446},{"$f":"'Example Analysis'!T133","v":11.074585287676445},{"$f":"'Example Analysis'!U133","v":3.2162035371499789}]
[]
[null,null,"Library ave concentrations and yields"]
[]
[null,null,"Sample name","Ave Library Concentration (pM)","Ave Library Concentration (nM)","Stnd Dev of Library Conc (nM)","Ave Conc of library (ng/µL)","Library volume (µL)","Available amount of library (ng)"]
[null,null,{"$f":"C5","v":"Connect_1"},{"$f":"AVERAGE(D5,D13)","v":12421.808605478109},{"$f":"AVERAGE(E5,E13)","v":12.421808605478109},{"$f":"STDEV(E5,E13)","v":0.39996259154508151},{"$f":"AVERAGE(F5,F13)","v":3.5767529603934132},{"$f":"25","v":25},{"$f":"G25*H25","v":89.418824009835333}]
[null,null,{"$f":"C6","v":"Connect_2"},8966.6114346939848,8.9666114346939843,{"$f":"STDEV(E6,E14)","v":0.44425928386520347},2.5375348961178155,{"$f":"25","v":25},{"$f":"G26*H26","v":63.43837240294539}]
[null,null,"Connect_3",11801.955786048024,11.801955786048026,0.46340922102518628,3.3399322439311758,{"$f":"25","v":25},83.4983060982794]
[null,null,"Connect_4",10651.834992432507,10.651834992432505,0.18941048833555596,3.0605225114481809,25,76.513062786204529]
[null,null,"Connect_5",13386.124005128011,13.386124005128011,0.49520976214140161,3.8626905726329346,25,96.567264315823365]
[null,null,"Connect_6",9983.9521618969229,9.9839521618969229,0.20939033125903211,2.8994694991929713,25,72.486737479824285]
[null,null,"Connect_7",11132.975717601963,11.132975717601962,0.3155276749072819,3.1987655485964073,25,79.969138714910187]
[null,null,"Connect_8",11225.439549347549,11.225439549347549,0.21334014279705402,3.2600135758446691,25,81.500339396116729]